diff --git a/.github/workflows/tests.yml b/.github/workflows/tests.yml
index ac045c0d7..25b9f102d 100644
--- a/.github/workflows/tests.yml
+++ b/.github/workflows/tests.yml
@@ -197,58 +197,24 @@ jobs:
     needs: download_data
     steps:
       - uses: actions/checkout@3d3c42e5aac5ba805825da76410c181273ba90b1  # v7.0.1
-      - name: Setup Miniconda
-        uses: conda-incubator/setup-miniconda@v4.0.1
+      - uses: prefix-dev/setup-pixi@f00437f565399d418b0acc85936d12c1fb668347  # v0.10.1
         with:
-          miniforge-version: latest
-          use-mamba: true
-          channels: conda-forge
-          channel-priority: strict
-          python-version: "3.12"
+          environments: "test-py313-pyside6"
 
-      - uses: tlambert03/setup-qt-libs@v1
+      - name: Setup headless display
+        uses: pyvista/setup-headless-display-action@5bc8de3bc71fcda7a96439571287a554901541a0 # v4.3
+        with:
+          qt: true
+          wm: herbstluftwm
 
       - name: Download test data
-        uses: actions/download-artifact@v8
+        uses: actions/download-artifact@3e5f45b2cfb9172054b4087a40e8e0b5a5461e7c # v8.0.1
         with:
           name: test_data
           path: test_data
 
-      - name: Use mamba solver
-        shell: "bash -el {0}"
-        run: |
-          conda install -n base conda-libmamba-solver
-          conda config --set solver libmamba
-
-      - name: Install dependencies
-        shell: "bash -el {0}"
-        run: |
-          mamba install -y "tox-conda>=0.10.0"
-
-      - name: List environment packages
-        shell: "bash -el {0}"
-        run: conda list
-
-      - name: create environment
-        shell: "bash -el {0}"
-        run: bash build_utils/create_environment_yml.sh
-
-      - name: Upload environment file
-        uses: actions/upload-artifact@v7
-        with:
-          name: environment
-          path: environment.yml
-          retention-days: 5
-
-      - name: patch tox.ini
-        shell: "bash -el {0}"
-        run: sed -e "s/{sys_platform}/{platform}/g" tox.ini -i
-
-      - name: Test with tox
-        uses: aganders3/headless-gui@v2
-        with:
-          run: conda run -n test --no-capture-output tox -e py312-PySide2-conda
-        timeout-minutes: 60
+      - name: Run tests
+        run: pixi run -e test-py313-pyside6 test
 
   check-package:
     name: Build & inspect our package.
diff --git a/.gitignore b/.gitignore
index b9f66fd5c..f5cb6b550 100644
--- a/.gitignore
+++ b/.gitignore
@@ -1,3 +1,4 @@
+pixi.lock
 report-*.json
 environment.yml
 minimum-constrains.txt
diff --git a/MANIFEST.in b/MANIFEST.in
index 32976046d..9a5d865b7 100644
--- a/MANIFEST.in
+++ b/MANIFEST.in
@@ -47,3 +47,4 @@ exclude runtime.txt
 exclude .sourcery.yaml
 exclude survey_url.txt
 exclude .typos.toml
+exclude pixi.toml
diff --git a/build_utils/create_environment_yml.sh b/build_utils/create_environment_yml.sh
deleted file mode 100644
index c003ef38f..000000000
--- a/build_utils/create_environment_yml.sh
+++ /dev/null
@@ -1,15 +0,0 @@
-#!/usr/bin/env bash
-
-set -euo pipefail
-
-dependencies=$(python build_utils/pyproject_toml_to_yaml.py)
-
-cat <<EOF > environment.yml
-name: test
-channels:
-  - conda-forge
-dependencies:
-$dependencies
-  - pyqt
-  - python=3.12
-EOF
diff --git a/build_utils/pyproject_toml_to_yaml.py b/build_utils/pyproject_toml_to_yaml.py
deleted file mode 100644
index 9edbbea78..000000000
--- a/build_utils/pyproject_toml_to_yaml.py
+++ /dev/null
@@ -1,31 +0,0 @@
-from pathlib import Path
-
-import tomllib
-
-base_dir = Path(__file__).parent.parent
-pyproject_toml = base_dir / "pyproject.toml"
-
-
-def drop_line(line):
-    if "python_version < '3.10'" in line:
-        return False
-
-    if "python_version < '3.11'" in line:  # noqa: SIM103
-        return False
-
-    return True
-
-
-def remove_specifier(line: str):
-    if ";" in line:
-        return line.split(";", maxsplit=1)[0]
-    return line
-
-
-with pyproject_toml.open("rb") as f:
-    data = tomllib.load(f)
-dependencies = data["project"]["dependencies"]
-dependencies = map(remove_specifier, filter(drop_line, dependencies))
-dependencies_str = "\n".join([f"  - {v}" for v in dependencies])
-dependencies_str = dependencies_str.replace("qtconsole", "qtconsole-base")
-print(dependencies_str)
diff --git a/pixi.toml b/pixi.toml
new file mode 100644
index 000000000..0e966dc8c
--- /dev/null
+++ b/pixi.toml
@@ -0,0 +1,70 @@
+[workspace]
+authors = ["Grzegorz Bokota"]
+channels = ["https://prefix.dev/conda-forge"]
+platforms = ["win-64", "linux-64", "osx-arm64"]  # Mac Intel support is not available
+preview = ["pixi-build"]
+
+[package]
+version = "0.17.1" # keep in sync with `pyproject.toml`
+
+[package.build]
+backend.name = "pixi-build-python"
+config.ignore-pypi-mapping = false
+
+[dependencies]
+napari = ">=0.7.0"
+
+[feature.py311.dependencies]
+python = "3.11.*"
+
+[feature.py312.dependencies]
+python = "3.12.*"
+
+[feature.py313.dependencies]
+python = "3.13.*"
+
+[feature.py314.dependencies]
+python = "3.14.*"
+
+[feature.pyqt6.dependencies]
+PartSeg = { path = ".", extras = ["pyqt6"] }
+
+[feature.pyside6.dependencies]
+PartSeg = { path = ".", extras = ["pyside6"] }
+
+[feature.test.dependencies]
+pytest = "*"
+pytest-qt = "*"
+pytest-timeout = "*"
+scikit-image = "*"
+lxml = "*"
+
+
+
+# there is no automatic mapping from `optional-dependencies`/`dependency-groups`
+# to `package.extra-dependencies`/`dependencies` yet,
+# ref. https://github.com/prefix-dev/pixi/issues/4764#issuecomment-4781239118
+[package.extra-dependencies.pyqt6]
+pyqt6 = ">=6.10"
+
+[package.extra-dependencies.pyside6]
+pyside6 = ">=6.10"
+
+
+
+
+[feature.test.tasks]
+test = "python -m pytest package/tests"
+
+[environments]
+default = { features = ["py312", "pyside6", "test"] }
+
+# Standard python x backend
+test-py311-pyqt6 = ["py311", "pyqt6", "test"]
+test-py312-pyqt6 = ["py312", "pyqt6", "test"]
+test-py313-pyqt6 = ["py313", "pyqt6", "test"]
+test-py314-pyqt6 = ["py314", "pyqt6", "test"]
+test-py311-pyside6 = ["py311", "pyside6", "test"]
+test-py312-pyside6 = ["py312", "pyside6", "test"]
+test-py313-pyside6 = ["py313", "pyside6", "test"]
+test-py314-pyside6 = ["py314", "pyside6", "test"]
diff --git a/tox.ini b/tox.ini
index e618bf690..b9cd15164 100644
--- a/tox.ini
+++ b/tox.ini
@@ -67,14 +67,6 @@ extras =
 commands =
     python -m pytest --json-report --json-report-file={toxinidir}/report-{envname}-{sys_platform}.json {posargs}
 
-[testenv:py312-PySide2-conda]
-conda_env=environment.yml
-deps=
-    pytest
-    pytest-json-report
-    lxml_html_clean
-
-
 [testenv:py{310,311,312,313,314,315}-{PyQt5,PySide2,PyQt6,PySide6}-napari_{62,70}]
 deps =
     {[testenv]deps}