"Syntrophobacter fumaroxidans MPOB T (DSM 10017) was cultivated under anoxic conditions in basal medium as described previously"
+"Syntrophobacter fumaroxidans MPOBT (DSM 10017) was cultivated under anoxic conditions in basal medium as described previously"
@@ -1084,7 +1084,7 @@"Cocultures of S. fumaroxidans with Methanospirillum hungatei strain JF1 T (DSM 864) or Methanobacterium formicicum MF T (DSM 1535) were grown with 30 mM of propionate without electron acceptor"
+"Cocultures of S. fumaroxidans with Methanospirillum hungatei strain JF1T (DSM 864) or Methanobacterium formicicum MFT (DSM 1535) were grown with 30 mM of propionate without electron acceptor"
diff --git a/kb/communities/Syntrophobacter_Methanobacterium_Syntrophy.yaml b/kb/communities/Syntrophobacter_Methanobacterium_Syntrophy.yaml index 8067e882..8728e37a 100644 --- a/kb/communities/Syntrophobacter_Methanobacterium_Syntrophy.yaml +++ b/kb/communities/Syntrophobacter_Methanobacterium_Syntrophy.yaml @@ -250,6 +250,38 @@ ecological_interactions: were used in its sulfidogenic metabolism explanation: Confirms the importance of formate as an interspecies electron carrier in addition to H2 +cultivation_setup: +- cultivation_mode: BATCH + system_type: FLASK + working_volume: 550.0 + working_volume_unit: mL + operating_temperature: 37.0 + operating_temperature_unit: °C + instrument_detail: >- + 1 L flasks holding 550 mL of medium, in triplicate, under a pressurised N2/CO2 (80:20) + gas phase at 172 kPa. The headspace is not just an anaerobic blanket: syntrophic + propionate oxidation is thermodynamically feasible only while the partner keeps H2 and + formate low, so the gas composition and pressure are part of the experiment. + notes: >- + The cited sentence says "all organisms", which in this paper covers the cocultures as + well as the axenic controls - so these are the community's conditions and not a member's. + That is the #529 check passing on the wording rather than on a member-name count, as it + did for the JN4/GD17 record. + + What is NOT recorded: the 20 mM propionate and 60 mM fumarate supplement, which the + source attaches specifically to "the medium for the pure cultures". Fumarate is an + electron acceptor that lets S. fumaroxidans grow without a partner, so carrying it into + the coculture's setup would describe a condition that removes the syntrophy this record + exists to document. + evidence: + - reference: PMID:29611893 + supports: SUPPORT + evidence_source: IN_VITRO + snippet: All organisms were batch cultured in triplicate at 37°C in 1 l flasks with 550 ml + medium under anaerobic conditions provided by a pressurised (172 kPa; 1.7 atm) gas phase + of N2/CO2 (80:20, vol/vol) + explanation: Mode, vessel, working volume, temperature and the pressurised anaerobic headspace. + environmental_factors: - name: Exogenous Formate Dosage value: 5-10 mM promotes; 50 mM inhibits @@ -393,7 +425,7 @@ growth_media: - reference: PMID:29611893 supports: SUPPORT evidence_source: IN_VITRO - snippet: Syntrophobacter fumaroxidans MPOB T (DSM 10017) was cultivated under anoxic conditions + snippet: Syntrophobacter fumaroxidans MPOBT (DSM 10017) was cultivated under anoxic conditions in basal medium as described previously explanation: Establishes anoxic basal-medium cultivation for the propionate-oxidizing partner. - reference: PMID:29611893 @@ -404,8 +436,8 @@ growth_media: - reference: PMID:29611893 supports: SUPPORT evidence_source: IN_VITRO - snippet: Cocultures of S. fumaroxidans with Methanospirillum hungatei strain JF1 T (DSM 864) or - Methanobacterium formicicum MF T (DSM 1535) were grown with 30 mM of propionate without electron + snippet: Cocultures of S. fumaroxidans with Methanospirillum hungatei strain JF1T (DSM 864) or + Methanobacterium formicicum MFT (DSM 1535) were grown with 30 mM of propionate without electron acceptor explanation: Confirms the S. fumaroxidans-M. formicicum coculture and propionate substrate concentration. discussions: