diff --git a/kb/communities/Rifle_Aquifer_Bioanode_EET_Community.yaml b/kb/communities/Rifle_Aquifer_Bioanode_EET_Community.yaml index a056f10c..de5b40d5 100644 --- a/kb/communities/Rifle_Aquifer_Bioanode_EET_Community.yaml +++ b/kb/communities/Rifle_Aquifer_Bioanode_EET_Community.yaml @@ -280,6 +280,41 @@ ecological_interactions: in the Rifle aquifer that may have the potential to mediate mineral redox transformations explanation: Supports the selective enrichment of EET-capable organisms. +cultivation_setup: +- system_type: BIOELECTROCHEMICAL_SYSTEM + instrument_detail: >- + Electroactive biofilms grown on poised anodes and maintained for almost four years, with + acetate as the sole carbon source. Both the anode biofilm and the planktonic fraction + were sampled, so the electrode is not merely a probe - it is the growth surface that + selects the community this record describes. + electrode_detail: >- + Anodes poised mostly at -0.2 to -0.25 V versus the standard hydrogen electrode, a range + chosen to mimic the redox potential of iron-oxide minerals. + notes: >- + `applied_potential` is left unset and the value kept in `electrode_detail`. The source + gives a range rather than a setpoint, and qualifies it with "mostly", so a single number + would assert a poise the four-year run did not hold. + + This is also the case #517 anticipated: the potential is only meaningful against its + reference electrode, and "vs SHE" is not a unit. Keeping the reference in + `electrode_detail` is what lets the range be stated in full rather than compressed into + a value-plus-suffix. + + No temperature or working volume - the source characterises the communities and does not + restate the reactor's dimensions. + + Predicted curatable in #543 on the grounds that a bioanode implies an electrochemical + cell, and it is: the biofilms were established and maintained rather than sampled from + the aquifer directly. + evidence: + - reference: PMID:32849356 + supports: SUPPORT + evidence_source: IN_VITRO + snippet: Electroactive biofilms were established and maintained for almost 4 years on anodes + poised mostly at -0.2 to -0.25 V vs. SHE, a range that mimics the redox potential of + iron-oxide minerals, using acetate as the sole carbon source + explanation: The electrode poise and its reference, the duration, and the sole carbon source. + environmental_factors: - name: Anode redox potential value: -0.2 to -0.25 V vs SHE