From 6a5a4006d3e18a2a376f2ac0ead3610350f92fd9 Mon Sep 17 00:00:00 2001 From: Don Freed Date: Thu, 9 Apr 2026 13:58:44 -0700 Subject: [PATCH 01/22] Replace `sentieon pyexec` with `sys.executable` - Add vcflib dependency --- poetry.lock | 12 ++++++++++++ pyproject.toml | 1 + sentieon_cli/command_strings.py | 21 ++++++++------------- sentieon_cli/dnascope.py | 3 +-- sentieon_cli/sentieon_pangenome.py | 9 +++------ 5 files changed, 25 insertions(+), 21 deletions(-) diff --git a/poetry.lock b/poetry.lock index 0117c37..578898d 100644 --- a/poetry.lock +++ b/poetry.lock @@ -2654,6 +2654,18 @@ enabler = ["pytest-enabler (>=2.2)"] test = ["big-O", "jaraco.functools", "jaraco.itertools", "jaraco.test", "more_itertools", "pytest (>=6,!=8.1.*)", "pytest-ignore-flaky"] type = ["pytest-mypy"] +[[package]] +name = "vcflib" +version = "1.0.1" +description = "An open-source Python library for VCF parsing and manipulation" +optional = false +python-versions = "!=3.0.*,!=3.1.*,!=3.2.*,!=3.3.*,!=3.4.*,!=3.5.*,>=2.7,>=3.6" +groups = ["main"] +files = [ + {file = "vcflib-1.0.1-py3-none-any.whl", hash = "sha256:26437875d441926b9fc6de2ce455c3516e6d6a4cce2e5ce638a94b05c803e705"}, + {file = "vcflib-1.0.1.tar.gz", hash = "sha256:0b814ea000c54c9885d63713c2a631a82f4bac9fba0f42a2c9c57fa8bed04759"}, +] + [metadata] lock-version = "2.1" python-versions = ">= 3.11" diff --git a/pyproject.toml b/pyproject.toml index 4172df5..e7fe720 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -22,6 +22,7 @@ dependencies = [ "mappy (>=2.30,<3.0)", "scipy (>=1.17.1,<2.0.0)", "levenshtein (>=0.27.3,<0.28.0)", + "vcflib (>=1.0.1,<2.0.0)", ] [project.scripts] diff --git a/sentieon_cli/command_strings.py b/sentieon_cli/command_strings.py index 8269348..a6a9b30 100644 --- a/sentieon_cli/command_strings.py +++ b/sentieon_cli/command_strings.py @@ -8,6 +8,7 @@ import pathlib import shlex import subprocess as sp +import sys import typing from typing import Any, Dict, List, Optional, Union @@ -115,7 +116,7 @@ def cmd_pyexec_vcf_mod_haploid_patch( if tech.upper() not in ("HIFI", "ONT"): raise ValueError(f"Unsupported tech '{tech}', must be 'HIFI' or 'ONT'") - cmd = f"sentieon pyexec {kwargs['vcf_mod_py']} -t {cores} " + cmd = f"{sys.executable} {kwargs['vcf_mod_py']} -t {cores} " cmd += "haploid_patch " cmd += f"--patch1 {hap1_patch} --patch2 {hap2_patch}" if tech.upper() == "HIFI": @@ -138,8 +139,7 @@ def cmd_pyexec_vcf_mod_patch( """Patch DNAscope and DNAscopeHP VCF files""" cmd = [ - "sentieon", - "pyexec", + sys.executable, str(kwargs["vcf_mod_py"]), "-t", str(cores), @@ -163,8 +163,7 @@ def cmd_pyexec_gvcf_combine( """Combine gVCF files""" cmd1 = Command( - "sentieon", - "pyexec", + sys.executable, str(kwargs["gvcf_combine_py"]), "-t", str(cores), @@ -196,8 +195,7 @@ def cmd_pyexec_vcf_mod_merge( """Merge haploid VCF files""" cmd = [ - "sentieon", - "pyexec", + sys.executable, kwargs["vcf_mod_py"], "-t", str(cores), @@ -227,8 +225,7 @@ def cmd_pyexec_vcf_mod_haploid_patch2( """Patch a single pair of haploid DNAscope and DNAscopeHP VCFs""" cmd = [ - "sentieon", - "pyexec", + sys.executable, str(kwargs["vcf_mod_py"]), "-t", str(cores), @@ -252,8 +249,7 @@ def cmd_pyexec_hybrid_select( slop_size: int = 1000, ) -> Pipeline: select_cmd = Command( - "sentieon", - "pyexec", + sys.executable, str(hybrid_select), "-v", str(vcf), @@ -302,8 +298,7 @@ def cmd_pyexec_hybrid_anno( threads: int, ) -> Pipeline: cmd = [ - "sentieon", - "pyexec", + sys.executable, str(hybrid_anno), "-v", str(vcf), diff --git a/sentieon_cli/dnascope.py b/sentieon_cli/dnascope.py index 09ed816..26dfd30 100644 --- a/sentieon_cli/dnascope.py +++ b/sentieon_cli/dnascope.py @@ -784,8 +784,7 @@ def dedup_and_metrics( rehead_job = Job( Pipeline( Command( - "sentieon", - "pyexec", + sys.executable, str(rehead_script), "--metrics_file", str(wgs_metrics), diff --git a/sentieon_cli/sentieon_pangenome.py b/sentieon_cli/sentieon_pangenome.py index 6f94ab3..7862766 100644 --- a/sentieon_cli/sentieon_pangenome.py +++ b/sentieon_cli/sentieon_pangenome.py @@ -869,8 +869,7 @@ def build_metrics_job( rehead_job = Job( Pipeline( Command( - "sentieon", - "pyexec", + sys.executable, str(rehead_script), "--metrics_file", str(wgs_metrics), @@ -1060,8 +1059,7 @@ def _build_indel2cnv_job( return Job( Pipeline( Command( - "sentieon", - "pyexec", + sys.executable, str(indel2cnv_script), str(self.reference), str(input_vcf), @@ -1087,8 +1085,7 @@ def _build_combine_cnv_job( return Job( Pipeline( Command( - "sentieon", - "pyexec", + sys.executable, str(combine_script), "--cnv", str(cnv_vcf), From f5b84a10dd3ba77af0f8144686c7f333fb683eb9 Mon Sep 17 00:00:00 2001 From: Don Freed Date: Thu, 9 Apr 2026 14:19:08 -0700 Subject: [PATCH 02/22] More informative errors for `bcftools` command failures --- sentieon_cli/shard.py | 8 ++++++++ sentieon_cli/util.py | 8 ++++++++ 2 files changed, 16 insertions(+) diff --git a/sentieon_cli/shard.py b/sentieon_cli/shard.py index ee1af4f..815f106 100644 --- a/sentieon_cli/shard.py +++ b/sentieon_cli/shard.py @@ -104,6 +104,14 @@ def vcf_contigs( kvpat = re.compile(r'(.*?)=(".*?"|.*?)(?:,|$)') cmd = ["bcftools", "view", "-h", str(in_vcf)] p = sp.run(cmd, capture_output=True, text=True) + if p.returncode != 0: + logger.error( + "`%s` failed with return code %d: %s", + " ".join(cmd), + p.returncode, + p.stderr.strip(), + ) + return {} contigs: Dict[str, Optional[int]] = {} for line in p.stdout.split("\n"): if not line.startswith("##contig"): diff --git a/sentieon_cli/util.py b/sentieon_cli/util.py index cf58108..0c02e4a 100644 --- a/sentieon_cli/util.py +++ b/sentieon_cli/util.py @@ -238,6 +238,14 @@ def vcf_id(in_vcf: pathlib.Path) -> Optional[str]: """Collect the SentieonVcfID header""" cmd = ["bcftools", "view", "-h", str(in_vcf)] p = sp.run(cmd, capture_output=True, text=True) + if p.returncode != 0: + logger.error( + "`%s` failed with return code %d: %s", + " ".join(cmd), + p.returncode, + p.stderr.strip(), + ) + return None for line in p.stdout.split("\n"): if line.startswith("##SentieonVcfID="): i = line.index("=") From 316b23056f05340dc115dd537f3fecfeb521d1a0 Mon Sep 17 00:00:00 2001 From: Don Freed Date: Fri, 10 Apr 2026 11:31:11 -0700 Subject: [PATCH 03/22] Updates to support additional pangenomes --- sentieon_cli/command_strings.py | 3 +++ sentieon_cli/sentieon_pangenome.py | 16 +++++++++++++++- 2 files changed, 18 insertions(+), 1 deletion(-) diff --git a/sentieon_cli/command_strings.py b/sentieon_cli/command_strings.py index a6a9b30..3e49970 100644 --- a/sentieon_cli/command_strings.py +++ b/sentieon_cli/command_strings.py @@ -1368,6 +1368,7 @@ def cmd_minimap2_lift( minimap2_i: str = "16G", threads=1, mm2_xargs: List[str] = [], + lift_prefix: str = "GRCh38#0#", ) -> Pipeline: """Minimap2 alignment with pgutil lift""" mm2_cmd = Command( @@ -1398,6 +1399,8 @@ def cmd_minimap2_lift( str(reference_fasta) + ".fai", "-g", str(gfa_file), + "--prefix", + lift_prefix, ) sort_cmd = Command( diff --git a/sentieon_cli/sentieon_pangenome.py b/sentieon_cli/sentieon_pangenome.py index 7862766..3b32e8e 100644 --- a/sentieon_cli/sentieon_pangenome.py +++ b/sentieon_cli/sentieon_pangenome.py @@ -110,6 +110,16 @@ class SentieonPangenome(BasePangenome): "help": "Generate a gVCF output file.", "action": "store_true", }, + "pangenome_ref_name": { + "default": "GRCh38", + "help": "Reference name in the pangenome (GRCh38)", + }, + "pangenome_contig_prefix": { + "default": "GRCh38#0#", + "help": ( + "Prefix to strip from pangenome contig names (GRCh38#0#)" + ), + }, "skip_metrics": { "help": "Skip metrics collection and multiQC", "action": "store_true", @@ -152,6 +162,8 @@ def __init__(self) -> None: self.bed: Optional[pathlib.Path] = None self.call_svs = False self.gvcf = False + self.pangenome_ref_name = "GRCh38" + self.pangenome_contig_prefix = "GRCh38#0#" self.skip_metrics = False self.skip_multiqc = False self.skip_contig_checks: bool = False @@ -680,7 +692,7 @@ def build_haplotypes_job( "--include-reference", "--diploid-sampling", "--set-reference", - "GRCh38", + self.pangenome_ref_name, ], ), "vg-haplotypes", @@ -697,6 +709,7 @@ def build_gfa_job( output_gfa, input_gbz, threads=self.cores, + reference_name=self.pangenome_ref_name, ), "vg-convert-gfa", 0, @@ -754,6 +767,7 @@ def build_minimap2_lift_job( "@RG\\t" + "\\t".join([f"{x[0]}:{x[1]}" for x in rg2.items()]), mm2_model, threads=self.cores, + lift_prefix=self.pangenome_contig_prefix, ), "mm2-lift", self.cores, From 6728fa42fe3e75f384019296cd4744e68ceb3888 Mon Sep 17 00:00:00 2001 From: Don Freed Date: Thu, 16 Apr 2026 13:10:48 -0700 Subject: [PATCH 04/22] Ensure the parent directory for the output file exists --- sentieon_cli/dnascope.py | 4 +--- sentieon_cli/dnascope_hybrid.py | 4 +--- sentieon_cli/dnascope_longread.py | 4 +--- sentieon_cli/pipeline.py | 8 ++++++++ 4 files changed, 11 insertions(+), 9 deletions(-) diff --git a/sentieon_cli/dnascope.py b/sentieon_cli/dnascope.py index 26dfd30..492e29a 100644 --- a/sentieon_cli/dnascope.py +++ b/sentieon_cli/dnascope.py @@ -278,9 +278,7 @@ def validate(self) -> None: "and `--readgroups` arguments" ) sys.exit(2) - if not str(self.output_vcf).endswith(".vcf.gz"): - self.logger.error("The output file should end with '.vcf.gz'") - sys.exit(2) + self.validate_output_vcf() self.skip_multiqc = True if self.skip_metrics else self.skip_multiqc if not library_preloaded("libjemalloc.so"): diff --git a/sentieon_cli/dnascope_hybrid.py b/sentieon_cli/dnascope_hybrid.py index 79184c1..9715c11 100644 --- a/sentieon_cli/dnascope_hybrid.py +++ b/sentieon_cli/dnascope_hybrid.py @@ -325,9 +325,7 @@ def validate(self) -> None: self.collect_readgroups() self.validate_readgroups() - if not str(self.output_vcf).endswith(".vcf.gz"): - self.logger.error("The output file should end with '.vcf.gz'") - sys.exit(2) + self.validate_output_vcf() if not self.sr_aln and not self.sr_r1_fastq: self.logger.error("Please supply a short-read input file") sys.exit(2) diff --git a/sentieon_cli/dnascope_longread.py b/sentieon_cli/dnascope_longread.py index 3261cc5..7181bdc 100644 --- a/sentieon_cli/dnascope_longread.py +++ b/sentieon_cli/dnascope_longread.py @@ -307,9 +307,7 @@ def validate(self) -> None: "files" ) sys.exit(2) - if not str(self.output_vcf).endswith(".vcf.gz"): - self.logger.error("The output file should end with '.vcf.gz'") - sys.exit(2) + self.validate_output_vcf() if self.tech.upper() == "ONT": self.logger.info("Skipping CNV calling with ONT data") self.skip_cnv = True diff --git a/sentieon_cli/pipeline.py b/sentieon_cli/pipeline.py index 68c4a03..8dc1455 100644 --- a/sentieon_cli/pipeline.py +++ b/sentieon_cli/pipeline.py @@ -160,6 +160,14 @@ def validate_output_vcf(self) -> None: if not str(self.output_vcf).endswith(".vcf.gz"): self.logger.error("The output file should end with '.vcf.gz'") sys.exit(2) + assert self.output_vcf is not None + parent = self.output_vcf.resolve().parent + if not parent.is_dir(): + self.logger.error( + "The parent directory of the output VCF does not exist: %s", + parent, + ) + sys.exit(2) def validate_ref(self) -> None: # Confirm the presence of the reference index file From fb716230a62e480b236ac4cbb1bb95530082b3a5 Mon Sep 17 00:00:00 2001 From: Don Freed Date: Fri, 17 Apr 2026 16:43:36 -0700 Subject: [PATCH 05/22] Validate bwa index files --- sentieon_cli/dnascope.py | 3 ++ sentieon_cli/dnascope_hybrid.py | 3 ++ sentieon_cli/pipeline.py | 19 ++++++++++++ sentieon_cli/sentieon_pangenome.py | 3 ++ tests/unit/test_pipeline_validation.py | 41 ++++++++++++++++++++++++++ 5 files changed, 69 insertions(+) diff --git a/sentieon_cli/dnascope.py b/sentieon_cli/dnascope.py index 492e29a..490a1a5 100644 --- a/sentieon_cli/dnascope.py +++ b/sentieon_cli/dnascope.py @@ -306,6 +306,9 @@ def validate(self) -> None: ) sys.exit(2) + if self.sr_r1_fastq or self.align or self.collate_align: + self.validate_bwa_index() + def configure(self) -> None: self.configure_alignment() diff --git a/sentieon_cli/dnascope_hybrid.py b/sentieon_cli/dnascope_hybrid.py index 9715c11..fc35c4e 100644 --- a/sentieon_cli/dnascope_hybrid.py +++ b/sentieon_cli/dnascope_hybrid.py @@ -352,6 +352,9 @@ def validate(self) -> None: ) sys.exit(2) + if self.sr_r1_fastq: + self.validate_bwa_index() + def validate_bundle(self) -> None: bundle_info_bytes = ar_load( str(self.model_bundle) + "/bundle_info.json" diff --git a/sentieon_cli/pipeline.py b/sentieon_cli/pipeline.py index 8dc1455..39672c1 100644 --- a/sentieon_cli/pipeline.py +++ b/sentieon_cli/pipeline.py @@ -25,6 +25,8 @@ "multiqc": packaging.version.Version("1.18"), } +BWA_INDEX_SUFFIXES = (".amb", ".ann", ".bwt", ".pac", ".sa") + class BasePipeline(ABC): """A pipeline base class""" @@ -180,6 +182,23 @@ def validate_ref(self) -> None: ) sys.exit(2) + def validate_bwa_index(self) -> None: + """Confirm the presence of BWA index files next to the reference.""" + missing = [ + str(self.reference) + suf + for suf in BWA_INDEX_SUFFIXES + if not os.path.isfile(str(self.reference) + suf) + ] + if missing: + self.logger.error( + "BWA index files are missing for reference %s: %s. Please " + "index the reference with 'sentieon bwa index %s'.", + self.reference, + ", ".join(missing), + self.reference, + ) + sys.exit(2) + @abstractmethod def configure(self) -> None: pass diff --git a/sentieon_cli/sentieon_pangenome.py b/sentieon_cli/sentieon_pangenome.py index 3b32e8e..e937403 100644 --- a/sentieon_cli/sentieon_pangenome.py +++ b/sentieon_cli/sentieon_pangenome.py @@ -221,6 +221,9 @@ def validate(self) -> None: ) sys.exit(2) + if self.r1_fastq: + self.validate_bwa_index() + if not self.skip_version_check: for cmd, min_version in SENT_PANGENOME_MIN_VERSIONS.items(): if not check_version(cmd, min_version): diff --git a/tests/unit/test_pipeline_validation.py b/tests/unit/test_pipeline_validation.py index 3585e07..b3ae579 100644 --- a/tests/unit/test_pipeline_validation.py +++ b/tests/unit/test_pipeline_validation.py @@ -43,6 +43,10 @@ def setup_method(self): for file_path in [self.mock_ref, self.mock_bam, self.mock_fastq, self.mock_bundle]: file_path.touch() + # Create BWA index files next to the reference + for suf in (".amb", ".ann", ".bwt", ".pac", ".sa"): + pathlib.Path(str(self.mock_ref) + suf).touch() + def test_valid_configuration_with_bam_input(self): """Test valid configuration with BAM input""" self.pipeline.output_vcf = self.mock_vcf @@ -443,5 +447,42 @@ def test_memory_calculation_logic(self): assert os.environ.get("bwt_max_mem") == "10G" +class TestValidateBwaIndex: + """Test BasePipeline.validate_bwa_index()""" + + BWA_SUFFIXES = (".amb", ".ann", ".bwt", ".pac", ".sa") + + def setup_method(self): + self.pipeline = DNAscopePipeline() + args = create_mock_args() + self.pipeline.setup_logging(args) + + self.temp_dir = tempfile.mkdtemp() + self.mock_ref = pathlib.Path(self.temp_dir) / "reference.fa" + self.mock_ref.touch() + self.pipeline.reference = self.mock_ref + + def _create_indexes(self, suffixes): + for suf in suffixes: + pathlib.Path(str(self.mock_ref) + suf).touch() + + def test_all_index_files_present(self): + self._create_indexes(self.BWA_SUFFIXES) + # Should not raise + self.pipeline.validate_bwa_index() + + def test_all_index_files_missing_raises(self): + with pytest.raises(SystemExit) as excinfo: + self.pipeline.validate_bwa_index() + assert excinfo.value.code == 2 + + def test_partial_index_files_missing_raises(self): + # All but .sa present + self._create_indexes(self.BWA_SUFFIXES[:-1]) + with pytest.raises(SystemExit) as excinfo: + self.pipeline.validate_bwa_index() + assert excinfo.value.code == 2 + + if __name__ == "__main__": pytest.main([__file__]) From 16b856219f2df9869f48e7a464af9e8c4e357215 Mon Sep 17 00:00:00 2001 From: Don Freed Date: Fri, 17 Apr 2026 22:11:02 -0700 Subject: [PATCH 06/22] Update test helpers --- tests/utils/test_helpers.py | 8 ++++++++ 1 file changed, 8 insertions(+) diff --git a/tests/utils/test_helpers.py b/tests/utils/test_helpers.py index 11e6a09..cfaf676 100644 --- a/tests/utils/test_helpers.py +++ b/tests/utils/test_helpers.py @@ -120,6 +120,10 @@ def create_dnascope_pipeline(self, **kwargs) -> DNAscopePipeline: # Create reference index self.fs.create_file("reference.fa.fai") + # Create BWA index files (required when aligning fastq or re-aligning) + for suf in (".amb", ".ann", ".bwt", ".pac", ".sa"): + self.fs.create_file("reference.fa" + suf) + # Default configuration pipeline.cores = 2 pipeline.dry_run = True @@ -198,6 +202,10 @@ def create_hybrid_pipeline(self, **kwargs) -> DNAscopeHybridPipeline: _fai = self.fs.create_file("reference.fa.fai") pipeline.model_bundle = self.fs.create_model_bundle("model.bundle", "DNAscope Hybrid") + # Create BWA index files (required when short-read fastq is supplied) + for suf in (".amb", ".ann", ".bwt", ".pac", ".sa"): + self.fs.create_file("reference.fa" + suf) + # Default configuration pipeline.cores = 2 pipeline.dry_run = True From fe4c97f44c737e06bd138c14bbebcf6eb35e0de9 Mon Sep 17 00:00:00 2001 From: Don Freed Date: Sat, 18 Apr 2026 09:50:18 -0700 Subject: [PATCH 07/22] More informative errors with invalid readgroups --- sentieon_cli/dnascope.py | 8 ++++++++ sentieon_cli/dnascope_hybrid.py | 11 ++++++++--- sentieon_cli/dnascope_longread.py | 7 +++++++ sentieon_cli/sentieon_pangenome.py | 8 +++++++- sentieon_cli/util.py | 19 +++++++++++++++++-- 5 files changed, 47 insertions(+), 6 deletions(-) diff --git a/sentieon_cli/dnascope.py b/sentieon_cli/dnascope.py index 490a1a5..c4fa230 100644 --- a/sentieon_cli/dnascope.py +++ b/sentieon_cli/dnascope.py @@ -44,6 +44,7 @@ from .util import ( check_version, library_preloaded, + parse_rg_line, path_arg, split_alignment, total_memory, @@ -306,6 +307,13 @@ def validate(self) -> None: ) sys.exit(2) + for rg in self.sr_readgroups: + try: + parse_rg_line(rg.replace(r"\t", "\t")) + except ValueError as e: + self.logger.error("Invalid --readgroups value '%s': %s", rg, e) + sys.exit(2) + if self.sr_r1_fastq or self.align or self.collate_align: self.validate_bwa_index() diff --git a/sentieon_cli/dnascope_hybrid.py b/sentieon_cli/dnascope_hybrid.py index fc35c4e..958095b 100644 --- a/sentieon_cli/dnascope_hybrid.py +++ b/sentieon_cli/dnascope_hybrid.py @@ -443,9 +443,14 @@ def collect_readgroups(self) -> None: self.all_readgroups[i][-1].append(parse_rg_line(rg_line)) for rg in self.sr_readgroups: self.all_readgroups[2].append([]) - self.all_readgroups[2][-1].append( - parse_rg_line(rg.replace(r"\t", "\t")) - ) + try: + parsed = parse_rg_line(rg.replace(r"\t", "\t")) + except ValueError as e: + self.logger.error( + "Invalid --sr_readgroups value '%s': %s", rg, e + ) + sys.exit(2) + self.all_readgroups[2][-1].append(parsed) def validate_readgroups(self) -> None: # Confirm that all readgroups have the same RGSM diff --git a/sentieon_cli/dnascope_longread.py b/sentieon_cli/dnascope_longread.py index 7181bdc..c256cd0 100644 --- a/sentieon_cli/dnascope_longread.py +++ b/sentieon_cli/dnascope_longread.py @@ -40,6 +40,7 @@ from .util import ( __version__, check_version, + parse_rg_line, path_arg, library_preloaded, vcf_id, @@ -307,6 +308,12 @@ def validate(self) -> None: "files" ) sys.exit(2) + for rg in self.readgroups: + try: + parse_rg_line(rg.replace(r"\t", "\t")) + except ValueError as e: + self.logger.error("Invalid --readgroups value '%s': %s", rg, e) + sys.exit(2) self.validate_output_vcf() if self.tech.upper() == "ONT": self.logger.info("Skipping CNV calling with ONT data") diff --git a/sentieon_cli/sentieon_pangenome.py b/sentieon_cli/sentieon_pangenome.py index e937403..89bbe36 100644 --- a/sentieon_cli/sentieon_pangenome.py +++ b/sentieon_cli/sentieon_pangenome.py @@ -395,7 +395,13 @@ def collect_readgroups(self) -> None: if not self.readgroup: return - parsed_rg = parse_rg_line(self.readgroup.replace(r"\t", "\t")) + try: + parsed_rg = parse_rg_line(self.readgroup.replace(r"\t", "\t")) + except ValueError as e: + self.logger.error( + "Invalid --readgroup value '%s': %s", self.readgroup, e + ) + sys.exit(2) if not parsed_rg.get("ID"): self.logger.error( "Readgroup '%s' does not have a RGID tag", diff --git a/sentieon_cli/util.py b/sentieon_cli/util.py index 0c02e4a..c8c3f8f 100644 --- a/sentieon_cli/util.py +++ b/sentieon_cli/util.py @@ -187,9 +187,24 @@ def split_alignment(cores: int) -> List[str]: def parse_rg_line(rg_line: str) -> Dict[str, str]: - """Parse an @RG line""" + """Parse an @RG line. + + Each tab-separated field after ``@RG`` must be of the form + ``KEY:VALUE``. Raises ``ValueError`` with the offending token if a + field lacks the ``KEY:`` prefix (e.g. a bare ``HG002`` instead of + ``SM:HG002``). + """ tags = rg_line.split("\t")[1:] - return {tag.split(":", 1)[0]: tag.split(":", 1)[1] for tag in tags} + parsed: Dict[str, str] = {} + for tag in tags: + key, sep, value = tag.partition(":") + if not sep or not key: + raise ValueError( + f"malformed @RG field '{tag}' — expected 'KEY:VALUE' " + f"(e.g. 'SM:sample')" + ) + parsed[key] = value + return parsed def get_read_length_aln( From 9f46c50cd9d33fa58c7fd467fd0e3514f6d11804 Mon Sep 17 00:00:00 2001 From: Don Freed Date: Sat, 18 Apr 2026 13:16:04 -0700 Subject: [PATCH 08/22] Replace `scipy.signal.find_peaks` and `Levenshtein.ratio` with Python implementations --- poetry.lock | 1092 +---------------- pyproject.toml | 2 - sentieon_cli/_indel2cnv_utils.py | 113 ++ sentieon_cli/scripts/indel2cnv.py | 60 +- tests/unit/test_indel2cnv_utils.py | 167 +++ tests/unit/test_kmc_patch.py | 33 + ...test_sentieon_pangenome_kmc_integration.py | 70 ++ 7 files changed, 416 insertions(+), 1121 deletions(-) create mode 100644 sentieon_cli/_indel2cnv_utils.py create mode 100644 tests/unit/test_indel2cnv_utils.py create mode 100644 tests/unit/test_kmc_patch.py create mode 100644 tests/unit/test_sentieon_pangenome_kmc_integration.py diff --git a/poetry.lock b/poetry.lock index 578898d..e7056ed 100644 --- a/poetry.lock +++ b/poetry.lock @@ -459,190 +459,6 @@ files = [ {file = "iniconfig-2.3.0.tar.gz", hash = "sha256:c76315c77db068650d49c5b56314774a7804df16fee4402c1f19d6d15d8c4730"}, ] -[[package]] -name = "jinja2" -version = "3.1.6" -description = "A very fast and expressive template engine." -optional = false -python-versions = ">=3.7" -groups = ["container"] -markers = "python_full_version != \"3.14.1\"" -files = [ - 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-[package.extras] -dev = ["nbval", "pytest (>=6)", "pytest-asyncio (>=0.24)", "pytest-cov", "pytest-timeout"] -discord = ["requests"] -notebook = ["ipywidgets (>=6)"] -slack = ["slack-sdk"] -telegram = ["requests"] - -[[package]] -name = "typeguard" -version = "4.5.1" -description = "Run-time type checker for Python" -optional = false -python-versions = ">=3.9" -groups = ["container"] -markers = "python_full_version != \"3.14.1\"" -files = [ - {file = "typeguard-4.5.1-py3-none-any.whl", hash = "sha256:44d2bf329d49a244110a090b55f5f91aa82d9a9834ebfd30bcc73651e4a8cc40"}, - {file = "typeguard-4.5.1.tar.gz", hash = "sha256:f6f8ecbbc819c9bc749983cc67c02391e16a9b43b8b27f15dc70ed7c4a007274"}, -] - -[package.dependencies] -typing_extensions = ">=4.14.0" - [[package]] name = "typing-extensions" version = "4.15.0" @@ -2669,4 +1579,4 @@ files = [ [metadata] lock-version = "2.1" python-versions = ">= 3.11" -content-hash = "76a7a9eb1f2f55ae3e55005a34dc6419eee843b1d8d4c1788179c13fa0e220c5" +content-hash = "c0805788407fac6a66aff9af2f102836ba48c4e87a99775602ef4f5abb97967c" diff --git a/pyproject.toml b/pyproject.toml index e7fe720..f71f426 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -20,8 +20,6 @@ include = ["LICENSE"] dependencies = [ "packaging (>=23.2)", "mappy (>=2.30,<3.0)", - "scipy (>=1.17.1,<2.0.0)", - "levenshtein (>=0.27.3,<0.28.0)", "vcflib (>=1.0.1,<2.0.0)", ] diff --git a/sentieon_cli/_indel2cnv_utils.py b/sentieon_cli/_indel2cnv_utils.py new file mode 100644 index 0000000..f366cbd --- /dev/null +++ b/sentieon_cli/_indel2cnv_utils.py @@ -0,0 +1,113 @@ +"""Pure-Python replacements for ``Levenshtein.ratio`` and +``scipy.signal.find_peaks`` used by ``scripts/indel2cnv.py``. + +Kept in the package (not ``scripts/``) so pytest can import them — +``scripts/`` is excluded from collection by ``conftest.py``. +""" + +from __future__ import annotations + +from collections import Counter +from typing import Sequence + + +def ratio(a: str, b: str, threshold: float = 0.0) -> float: + """Return ``2 * LCS(a, b) / (len(a) + len(b))``. + + Equivalent to ``Levenshtein.ratio(a, b)`` (which uses insert/delete + cost 1 and substitution cost 2, making the underlying distance + equal to ``|a| + |b| - 2 * LCS(a, b)``). + + When ``threshold > 0`` and a cheap upper bound proves the ratio + cannot reach ``threshold``, returns ``0.0`` as a sentinel without + running the full LCS. Otherwise returns the exact ratio. + """ + la, lb = len(a), len(b) + if la == 0 and lb == 0: + return 1.0 + if la == 0 or lb == 0: + return 0.0 + if a == b: + return 1.0 + + tot = la + lb + + # Length-based upper bound: 2*LCS <= 2*min(|a|,|b|). + if threshold > 0.0 and 2 * min(la, lb) < threshold * tot: + return 0.0 + + # Character-multiset intersection is a true upper bound on 2*LCS. + ub_matches = sum((Counter(a) & Counter(b)).values()) + if ub_matches == 0: + return 0.0 + if threshold > 0.0 and (2.0 * ub_matches) < threshold * tot: + return 0.0 + + return 2.0 * _bit_parallel_lcs(a, b) / tot + + +def _bit_parallel_lcs(a: str, b: str) -> int: + """LCS length via the Allison-Dix bit-parallel algorithm. + + Each iteration is O(len(a) / word_size) big-int operations; Python + int ops exploit this implicitly. Puts the shorter string in ``a`` + to minimize bitmap width. + """ + if len(a) > len(b): + a, b = b, a + m = len(a) + + char_mask: dict[str, int] = {} + for i, ch in enumerate(a): + char_mask[ch] = char_mask.get(ch, 0) | (1 << i) + + full = (1 << m) - 1 + s = full + for ch in b: + t = char_mask.get(ch) + if t is None: + continue + u = s & t + if u == 0: + continue + s = ((s + u) | (s - u)) & full + return m - bin(s).count("1") + + +def find_peaks( + values: Sequence[float], *, distance: int, height: float +) -> list[int]: + """Return indices of local maxima >= ``height`` with no two kept + peaks closer than ``distance`` samples. + + Matches ``scipy.signal.find_peaks(x, distance=d, height=h)`` for the + one call site that uses it. Plateaus map to their midpoint index + (``(start + end) // 2``), matching scipy's behavior. Boundary + values are never peaks. + """ + n = len(values) + candidates: list[int] = [] + i = 1 + while i < n - 1: + if values[i] <= values[i - 1] or values[i] < height: + i += 1 + continue + j = i + while j + 1 < n and values[j + 1] == values[i]: + j += 1 + if j + 1 < n and values[j + 1] < values[i]: + candidates.append((i + j) // 2) + i = j + 1 + + if distance <= 1 or len(candidates) <= 1: + return candidates + + # Keep highest peaks first; drop any within `distance` of one + # already kept. Tie-break on index so earlier wins. + ordered = sorted(candidates, key=lambda k: (-values[k], k)) + kept: list[int] = [] + for p in ordered: + if all(abs(p - q) >= distance for q in kept): + kept.append(p) + kept.sort() + return kept diff --git a/sentieon_cli/scripts/indel2cnv.py b/sentieon_cli/scripts/indel2cnv.py index c5fe617..9419dbb 100755 --- a/sentieon_cli/scripts/indel2cnv.py +++ b/sentieon_cli/scripts/indel2cnv.py @@ -1,9 +1,7 @@ import collections -import Levenshtein import math import io import vcflib -from scipy.signal import find_peaks from multiprocessing import Pool import argparse import logging @@ -13,8 +11,14 @@ import platform import mappy import os +import pathlib import re +# When launched as `python indel2cnv.py ...` from an arbitrary CWD, +# `sentieon_cli` is only importable if the package root is on sys.path. +sys.path.insert(0, str(pathlib.Path(__file__).resolve().parents[2])) +from sentieon_cli._indel2cnv_utils import find_peaks, ratio # noqa: E402 + # Convert long INDELs (from assembly-based SV calls) into tandem CNV truth events. # Pipeline: find_repeats (detect period) -> match_ref (locate repeat on reference) # -> extend array boundaries -> emit DEL/DUP with REFSTART/REFSTOP for cnv_eval. @@ -96,13 +100,13 @@ def find_repeats(var_seq, thresh): step = 200 if length > 2000 else 100 start = step * 2 scan_limit = min(length//2+step*5, length-step*1) - dist = [Levenshtein.ratio(var_seq[:n], var_seq[n:n*2]) for n in range(start, scan_limit, step)] + dist = [ratio(var_seq[:n], var_seq[n:n*2], threshold=thresh) for n in range(start, scan_limit, step)] if not dist or max(dist) < thresh: return [len(var_seq)] - peaks, _ = find_peaks(dist, distance=start//step, height=0.95) - if peaks.size == 0: + peaks = find_peaks(dist, distance=start//step, height=0.95) + if len(peaks) == 0: mid = length//2 - if Levenshtein.ratio(var_seq[:mid], var_seq[mid:mid*2]) > thresh: + if ratio(var_seq[:mid], var_seq[mid:mid*2], threshold=thresh) > thresh: peaks = [mid/step] else: peaks = [p+start//step for p in peaks] @@ -116,14 +120,14 @@ def find_repeats(var_seq, thresh): p_min, p_max = int(length/(cnt+0.5)), int(length/(cnt-0.5)) p_step = max(1, (p_max - p_min)//10) while True: - ratios = [max([Levenshtein.ratio(var_seq[i*p:(i+1)*p], var_seq[(i+1)*p:(i+2)*p]) for i in range(cnt-1)]) for p in range(p_min, p_max+1, p_step)] + ratios = [max([ratio(var_seq[i*p:(i+1)*p], var_seq[(i+1)*p:(i+2)*p], threshold=thresh) for i in range(cnt-1)]) for p in range(p_min, p_max+1, p_step)] best_ratio = max(ratios) best_period = p_min + (ratios.index(best_ratio))*p_step if p_step == 1: break p_min, p_max = best_period - 2 * p_step, best_period + 2 * p_step p_step = max(1, (p_max - p_min)//10) - ratio_orig = max([Levenshtein.ratio(var_seq[i*period:(i+1)*period], var_seq[(i+1)*period:(i+2)*period]) for i in range(cnt-1)]) + ratio_orig = max([ratio(var_seq[i*period:(i+1)*period], var_seq[(i+1)*period:(i+2)*period], threshold=thresh) for i in range(cnt-1)]) if best_ratio > ratio_orig: period = best_period periods = {period: max(best_ratio, ratio_orig)} @@ -134,7 +138,7 @@ def find_repeats(var_seq, thresh): break cur_cnt = int(round(length/cur_period)) if cur_cnt >= 2: - periods[cur_period] = max([Levenshtein.ratio(var_seq[j*cur_period:(j+1)*cur_period], var_seq[(j+1)*cur_period:(j+2)*cur_period]) for j in range(cur_cnt-1)]) + periods[cur_period] = max([ratio(var_seq[j*cur_period:(j+1)*cur_period], var_seq[(j+1)*cur_period:(j+2)*cur_period], threshold=thresh) for j in range(cur_cnt-1)]) i += 1 periods = {k: v for k, v in periods.items() if k >= MIN_SEQ_LEN} if len(periods) > 1: @@ -182,7 +186,7 @@ def mm2_match(seq, ref, chrom, pos, half_win, thresh): matched_ref_length = ref_stop - ref_start best_matched_ref = ref.get(chrom, ref_start, ref_stop) matched_seq = seq[seq_start:seq_stop] - best_ratio = Levenshtein.ratio(best_matched_ref, matched_seq) + best_ratio = ratio(best_matched_ref, matched_seq, threshold=thresh) if best_ratio < thresh: return None # Partial match extension: when mappy aligned only part of seq, the period boundary @@ -202,10 +206,10 @@ def mm2_match(seq, ref, chrom, pos, half_win, thresh): best_ext = 0 while True: for l in range(start_i, end_i, step): - ratio = Levenshtein.ratio(best_matched_ref + ref_after[:l], seq[seq_start:]+seq[:l-seq_len_diff]) - if ratio > thresh: + r = ratio(best_matched_ref + ref_after[:l], seq[seq_start:]+seq[:l-seq_len_diff], threshold=thresh) + if r > thresh: last_valid = l - last_valid_ratio = ratio + last_valid_ratio = r else: last_invalid = l break @@ -235,10 +239,10 @@ def mm2_match(seq, ref, chrom, pos, half_win, thresh): best_ext = 0 while True: for l in range(start_i, end_i, step): - ratio = Levenshtein.ratio(ref_before[-l:] + best_matched_ref, seq[-l+seq_start:] + seq[:seq_stop]) - if ratio > thresh: + r = ratio(ref_before[-l:] + best_matched_ref, seq[-l+seq_start:] + seq[:seq_stop], threshold=thresh) + if r > thresh: last_valid = l - last_valid_ratio = ratio + last_valid_ratio = r else: last_invalid = l break @@ -262,11 +266,11 @@ def mm2_match(seq, ref, chrom, pos, half_win, thresh): ref_stop_ext = ref_stop for _ in range(MAX_EXTEND_ITERS): ref_start_ext -= matched_ref_length - if ref_start_ext < 0 or Levenshtein.ratio(ref.get(chrom, ref_start_ext, ref_start_ext+matched_ref_length), best_matched_ref) < thresh: + if ref_start_ext < 0 or ratio(ref.get(chrom, ref_start_ext, ref_start_ext+matched_ref_length), best_matched_ref, threshold=thresh) < thresh: break for _ in range(MAX_EXTEND_ITERS): ref_stop_ext += matched_ref_length - if Levenshtein.ratio(ref.get(chrom, ref_stop_ext-matched_ref_length, ref_stop_ext), best_matched_ref) < thresh: + if ratio(ref.get(chrom, ref_stop_ext-matched_ref_length, ref_stop_ext), best_matched_ref, threshold=thresh) < thresh: break return (ref_start, ref_stop), (ref_start_ext, ref_stop_ext), (seq_start, seq_stop), best_ratio @@ -287,8 +291,8 @@ def local_match(v, ref, period, thresh): elif offset < 0: ref_after = ref_after[-offset:] alt_period_seq = alt_seq[:period] - ratios = {p: Levenshtein.ratio(ref_after[:p] + ref_before[-(period-p):], alt_period_seq) for p in range(0, period, step)} - ratios[period] = Levenshtein.ratio(ref_after, alt_period_seq) + ratios = {p: ratio(ref_after[:p] + ref_before[-(period-p):], alt_period_seq, threshold=0.8) for p in range(0, period, step)} + ratios[period] = ratio(ref_after, alt_period_seq, threshold=0.8) while True: best_pos = max(ratios, key=ratios.get) max_ratio = ratios[best_pos] @@ -301,7 +305,7 @@ def local_match(v, ref, period, thresh): p_hi = min(best_pos + step, period) for p in (p_lo, p_hi): if p not in ratios: - ratios[p] = Levenshtein.ratio(ref_after[:p] + ref_before[-(period-p):], alt_period_seq) + ratios[p] = ratio(ref_after[:p] + ref_before[-(period-p):], alt_period_seq, threshold=0.8) if max_ratio < thresh: return None ref_start = v.pos - (period - best_pos) @@ -316,11 +320,11 @@ def local_match(v, ref, period, thresh): ref_end_ext = ref_end for _ in range(MAX_EXTEND_ITERS): ref_start_ext -= period - if ref_start_ext < 0 or Levenshtein.ratio(ref.get(v.chrom, ref_start_ext, ref_start_ext+period), ref_seq) < thresh: + if ref_start_ext < 0 or ratio(ref.get(v.chrom, ref_start_ext, ref_start_ext+period), ref_seq, threshold=thresh) < thresh: break for _ in range(MAX_EXTEND_ITERS): ref_end_ext += period - if Levenshtein.ratio(ref.get(v.chrom, ref_end_ext-period, ref_end_ext), ref_seq) < thresh: + if ratio(ref.get(v.chrom, ref_end_ext-period, ref_end_ext), ref_seq, threshold=thresh) < thresh: break return (ref_start, ref_end), (ref_start_ext, ref_end_ext), (0, period), max_ratio @@ -350,11 +354,11 @@ def proc_variant(v, ref, thresh): if len(v.ref) > MIN_SEQ_LEN and len(v.ref) - len(a) > MIN_SEQ_LEN: v1 = copy_variant(v, alt=[a]) if len(a) > 1: - if a == v1.ref[:len(a)] or len(a) < 10 or Levenshtein.ratio(a, v1.ref[:len(a)]) > thresh: + if a == v1.ref[:len(a)] or len(a) < 10 or ratio(a, v1.ref[:len(a)], threshold=thresh) > thresh: v1.pos += len(a) - 1 v1.ref = v.ref[len(a):] v1.alt = [a[-1]] - elif Levenshtein.ratio(a, v.ref[-len(a):]) > thresh: + elif ratio(a, v.ref[-len(a):], threshold=thresh) > thresh: v1.ref = v.ref[:-len(a)] v1.alt = [v.ref[0]] v1.samples[0]['GT'] = '0/1' @@ -363,11 +367,11 @@ def proc_variant(v, ref, thresh): elif len(a) - len(v.ref) > MIN_SEQ_LEN: v1 = copy_variant(v) v1.samples[0]['GT'] = '0/1' - if len(v.ref) < 6 or Levenshtein.ratio(v.alt[i][:len(v.ref)], v.ref) > min(thresh, 1-5/len(v.ref)): + if len(v.ref) < 6 or ratio(v.alt[i][:len(v.ref)], v.ref, threshold=min(thresh, 1-5/len(v.ref))) > min(thresh, 1-5/len(v.ref)): v1.alt = [v1.alt[i][len(v.ref)-1:]] v1.pos += len(v.ref) - 1 v1.ref = v.ref[-1] - elif len(v.ref) < 6 or Levenshtein.ratio(v.alt[i][-len(v.ref):], v.ref) > min(thresh, 1-5/len(v.ref)): + elif len(v.ref) < 6 or ratio(v.alt[i][-len(v.ref):], v.ref, threshold=min(thresh, 1-5/len(v.ref))) > min(thresh, 1-5/len(v.ref)): v1.alt = [v1.alt[i][:-len(v.ref)]] v1.ref = v.ref[0] else: @@ -412,7 +416,7 @@ def proc_variant(v, ref, thresh): updated_periods = [] for p0 in periods[0]: p1s = [p1 for p1 in periods[1] if abs(p0/p1-1) <= 0.05] - if p1s and Levenshtein.ratio(*(a[:min(p0, p1s[0])] for a in v.alt)) > 1 - (1-thresh)/2: + if p1s and ratio(*(a[:min(p0, p1s[0])] for a in v.alt), threshold=1 - (1-thresh)/2) > 1 - (1-thresh)/2: updated_periods.append(min(p0, p1s[0])) if updated_periods: periods = [updated_periods] diff --git a/tests/unit/test_indel2cnv_utils.py b/tests/unit/test_indel2cnv_utils.py new file mode 100644 index 0000000..9722b96 --- /dev/null +++ b/tests/unit/test_indel2cnv_utils.py @@ -0,0 +1,167 @@ +"""Golden-value tests for ``sentieon_cli._indel2cnv_utils``. + +Expected values were captured from ``Levenshtein.ratio`` (v0.27.3) and +``scipy.signal.find_peaks`` (v1.17.1). Keeping the deps as test-time +imports would defeat the point of this module, so the reference +outputs are frozen as literals here. +""" + +import os +import sys + +sys.path.insert( + 0, + os.path.abspath(os.path.join(os.path.dirname(__file__), "..", "..")), +) + +import pytest + +from sentieon_cli._indel2cnv_utils import find_peaks, ratio + + +LONG_A = ( + "AAGCCCAATAAACCACTCTGACTGGCCGAATAGGGATATAGGCAACGACATGTGCGGCGACC" + "CTTGCGACAGTGACGCTTTCGCCGTTGCCTAAACCTATTTGAAGGAGTCTAGCAGCCGCAGT" + "AAGGCACAATACCTCGTCCGTGTTACCAGACCAAACAAGACGTCCTCTTCAATGTTTAAATG" + "ACCCTCTCGTCATAAAACCTTTCTACTATGTGTTCCGCAAGAATCAACAACTACAATGGCGC" + "GTCGTGAATAACGCGACGGCTGAGACGAACGGCGCGTGAATGAAGCGCTTAAACAGCTCAGG" + "AGCCAGTCCCCTACGTCGCATATCCTGGCCACTGGAGGTGAAGCGAATGGTATCGATACGTA" + "GGAGGTGTGCCTTCGTAGGCTGTTTCTCAGGACGCCCAACTATTCTTTCCAATCCTACATCT" + "GTTTCTTGCGTCGTAGCGGGACCCTCCATTGTTACTTATTAGGTTCTCGTTATGTCTCATAA" + "TCTC" +) +LONG_B = ( + "AAGCCCAATAAACCACTCTGCCTGGGGGAATAGGGTTATAGGCAACGACATGTGCGGGCTAC" + "CCTTGCGACAGTGACGCTTTCGCCAGTTGCCTAAACCTATTTAAAGGACTCTAGTAGCCGCA" + "ATAAGGCCCAATACCTCGTCGGTGTTACCAGACCAAACAAGACGTGCTCTTCAATGTTTAAA" + "TGACCCTCTCCTCATAAAACCTTTCTACTATGTGTTCCGCAAGAATCAACAACTGACCAGGG" + "CGCGTCGTGAATAACGCGACGGCTGAGACGAACGGCGCGTGAATGATAGCGCTTAAACAGCT" + "CAGGAGCCAGTCACCTACGTCGCATATCCTGGCCACTGGAGGTGAAGCGAATGGTATCGATA" + "CGTAGGAGGTGTGCCGTCGTACGCTGTTGTCTCAGGACGCCCAACTATTCTTTCCAATCCTA" + "CATCTGTTTCTTGCGTCGTAGCGGGACCCTCCATTGTTACTTATTAGGTTCTCGTTCAGTCT" + "CATAATCTC" +) +LONG_C = ( + "TCACAGAAGTGAGATTATGTCTCGTTTGGCAGTCTTGATGCTCGGGGGACACTTCTTTAAGC" + "TCGGTGTGGTGGGCACGACCCTGGACGCGCGACGAAGCTAAGTTTGCAGTAATTAACCGACA" + "TCTTTGTGAACCGACCCACATTTGACGGTACGCTACCGCAACGGTATGTGTTAATGGAACAG" + "ACTTGCTTATGTGGACGTTGTATAGGGATATTACGTTACGCGTTAACCGATACATACTGGTT" + "TCTCTCCAGTGGAGGTCTTGGTTGCCTCTAGTTTCTACGATATACTCATGGTAGTGTAACGC" + "ATAATCGAAGAGGGTCCTCCCATCTCCTGTGATGCATGGTGTGCTTACTGGGATGAATGCGC" + "CGCAAGTAGCAGGTCCCGGCGTGGATACCTGATAGATGGTGACTAGCATGTACAAGTAACCT" + "TGTCTATTGAGCTTCGAGGATGCATACAAGCCCACCCGCAGCCGCAACAGCGACGACTAATT" + "GATC" +) +RATIO_AB = 0.9572139303482587 +RATIO_AC = 0.636 + +TANDEM_UNIT = "ACGTACGAT" +TANDEM = TANDEM_UNIT * 6 +TANDEM_VAR = ( + TANDEM[: len(TANDEM) // 2] + "T" + TANDEM[len(TANDEM) // 2 + 1:] +) +TANDEM_RATIO = 0.9814814814814815 + + +class TestRatio: + """``ratio`` must match ``Levenshtein.ratio`` on the captured cases.""" + + @pytest.mark.parametrize( + "a,b,expected", + [ + ("ACGTACGT", "ACGTACGT", 1.0), + ("", "", 1.0), + ("ACGT", "", 0.0), + ("", "ACGT", 0.0), + ("AAAA", "CCCC", 0.0), + ("ACGT", "ACGGT", 0.8888888888888888), + ("ACGGT", "ACGT", 0.8888888888888888), + ("ACGT", "AGGT", 0.75), + ("ACGTACGT" * 4, "ACGTACGT" * 4, 1.0), + ("ACGTACGT" * 4, "CGTACGTA" * 4, 0.96875), + ("AACCGGTT", "AACCGGTG", 0.875), + (TANDEM, TANDEM_VAR, TANDEM_RATIO), + ], + ) + def test_golden_values(self, a, b, expected): + assert ratio(a, b) == pytest.approx(expected, abs=1e-12) + + def test_long_similar_dna(self): + assert ratio(LONG_A, LONG_B) == pytest.approx(RATIO_AB, abs=1e-12) + + def test_long_unrelated_dna(self): + assert ratio(LONG_A, LONG_C) == pytest.approx(RATIO_AC, abs=1e-12) + + def test_symmetry(self): + assert ratio(LONG_A, LONG_B) == ratio(LONG_B, LONG_A) + + def test_threshold_pass_returns_exact_value(self): + # Real ratio is above threshold — must return the exact value. + assert ratio(LONG_A, LONG_B, threshold=0.9) == pytest.approx( + RATIO_AB, abs=1e-12 + ) + + def test_threshold_fail_returns_below_threshold(self): + # Real ratio is below threshold — returned value must be < threshold. + result = ratio("AAAA", "CCCC", threshold=0.5) + assert result < 0.5 + + def test_threshold_zero_disables_abort(self): + # Whatever value threshold=0 returns must equal the no-threshold call. + assert ratio(LONG_A, LONG_C, threshold=0.0) == ratio(LONG_A, LONG_C) + + def test_threshold_very_asymmetric_lengths(self): + # Length-based upper bound rejects cheaply. + result = ratio("A" * 10, "A" * 10000, threshold=0.9) + assert result < 0.9 + + +class TestFindPeaks: + """``find_peaks`` must match ``scipy.signal.find_peaks(x, distance=d, + height=h)`` for the call pattern used by indel2cnv.py.""" + + @pytest.mark.parametrize( + "values,distance,height,expected", + [ + ([0.0, 0.5, 1.0, 0.5, 0.0], 1, 0.9, [2]), + ( + [0.0, 0.95, 0.0, 0.0, 0.0, 0.97, 0.0], + 2, 0.9, [1, 5], + ), + ([0.0, 0.95, 0.0, 0.97, 0.0], 3, 0.9, [3]), + ([0.0, 0.5, 0.0, 0.6, 0.0], 1, 0.9, []), + ([0.0, 0.95, 0.95, 0.0], 1, 0.9, [1]), + ([1.0, 0.9, 0.8], 1, 0.9, []), + ([0.8, 0.9, 1.0], 1, 0.9, []), + ( + [0.3, 0.5, 0.6, 0.95, 0.7, 0.6, 0.5, 0.96, 0.4, 0.3], + 2, 0.9, [3, 7], + ), + ], + ) + def test_golden_values(self, values, distance, height, expected): + assert find_peaks( + values, distance=distance, height=height + ) == expected + + def test_empty_input(self): + assert find_peaks([], distance=1, height=0.9) == [] + + def test_two_element_input(self): + # Neither element has both neighbors; never a peak. + assert find_peaks([0.0, 1.0], distance=1, height=0.5) == [] + + def test_left_edge_plateau_not_a_peak(self): + # scipy ignores plateaus without a strictly lower left neighbor. + assert find_peaks([0.95, 0.95, 0.0], distance=1, height=0.9) == [] + + def test_right_edge_plateau_not_a_peak(self): + # scipy ignores plateaus without a strictly lower right neighbor. + assert find_peaks([0.0, 0.95, 0.95], distance=1, height=0.9) == [] + + def test_wider_plateau_midpoint(self): + # Plateau of width 3 at indices 2..4; midpoint = 3. + assert find_peaks( + [0.0, 0.3, 0.95, 0.95, 0.95, 0.3, 0.0], + distance=1, height=0.9, + ) == [3] diff --git a/tests/unit/test_kmc_patch.py b/tests/unit/test_kmc_patch.py new file mode 100644 index 0000000..1589354 --- /dev/null +++ b/tests/unit/test_kmc_patch.py @@ -0,0 +1,33 @@ +""" +Tests for KMC patch check +""" + +import subprocess as sp +from unittest.mock import MagicMock, patch + +from sentieon_cli.util import check_kmc_patch + + +def test_check_kmc_patch_success(): + """Test check_kmc_patch returns True on success""" + with patch("subprocess.run") as mock_run: + mock_run.return_value.returncode = 0 + assert check_kmc_patch() is True + mock_run.assert_called_once() + args, kwargs = mock_run.call_args + assert "kmc" == args[0][0] + assert kwargs["input"] is not None + + +def test_check_kmc_patch_failure(): + """Test check_kmc_patch returns False on failure""" + with patch("subprocess.run") as mock_run: + mock_run.side_effect = sp.CalledProcessError(1, "kmc") + assert check_kmc_patch() is False + + +def test_check_kmc_patch_not_found(): + """Test check_kmc_patch returns False if kmc is missing""" + with patch("subprocess.run") as mock_run: + mock_run.side_effect = FileNotFoundError + assert check_kmc_patch() is False diff --git a/tests/unit/test_sentieon_pangenome_kmc_integration.py b/tests/unit/test_sentieon_pangenome_kmc_integration.py new file mode 100644 index 0000000..cfe4e61 --- /dev/null +++ b/tests/unit/test_sentieon_pangenome_kmc_integration.py @@ -0,0 +1,70 @@ +""" +Tests for KMC patch integration in SentieonPangenome +""" + +import sys +import unittest +from unittest.mock import MagicMock, patch + +from sentieon_cli.sentieon_pangenome import SentieonPangenome + + +class TestSentieonPangenomeKMCIntegration(unittest.TestCase): + def test_validate_kmc_patch_success(self): + """Test validation passes when KMC patch check succeeds""" + pipeline = SentieonPangenome() + pipeline.sample_input = ["sample.bam"] + pipeline.skip_version_check = False + pipeline.skip_pangenome_name_checks = True # skip irrelevant checks + pipeline.skip_contig_checks = True + pipeline.bed = "bed" # mock existence + pipeline.reference = "ref" + pipeline.pop_vcf = "pop" + pipeline.gbz = "ref.grch38.gbz" + pipeline.fai_data = {} + pipeline.pop_vcf_contigs = {} + + # Mock dependencies + pipeline.handle_arguments = MagicMock() + pipeline.setup_logging = MagicMock() + pipeline.validate_bundle = MagicMock() + pipeline.validate_fastq_rg = MagicMock() + pipeline.validate_output_vcf = MagicMock() + pipeline.validate_ref = MagicMock() + pipeline.collect_readgroups = MagicMock() + + with patch("sentieon_cli.sentieon_pangenome.check_version", return_value=True), \ + patch("sentieon_cli.sentieon_pangenome.check_kmc_patch", return_value=True) as mock_check, \ + patch("sys.exit") as mock_exit: + + pipeline.validate() + + mock_check.assert_called_with("kmc") + mock_exit.assert_not_called() + + def test_validate_kmc_patch_failure(self): + """Test validation fails when KMC patch check fails""" + pipeline = SentieonPangenome() + pipeline.sample_input = ["sample.bam"] + pipeline.skip_version_check = False + # other attributes mock + pipeline.validate_bundle = MagicMock() + pipeline.validate_fastq_rg = MagicMock() + pipeline.validate_output_vcf = MagicMock() + pipeline.validate_ref = MagicMock() + pipeline.collect_readgroups = MagicMock() + pipeline.bed = "bed" + pipeline.fai_data = {} + pipeline.pop_vcf_contigs = {} + + pipeline.logger = MagicMock() + + with patch("sentieon_cli.sentieon_pangenome.check_version", return_value=True), \ + patch("sentieon_cli.sentieon_pangenome.check_kmc_patch", return_value=False) as mock_check, \ + patch("sys.exit") as mock_exit: + + pipeline.validate() + + mock_check.assert_called_with("kmc") + pipeline.logger.error.assert_called() + mock_exit.assert_called_with(2) From 4e8563860c9774df904914f27cc89b9c0cc2f2a8 Mon Sep 17 00:00:00 2001 From: Don Freed Date: Sun, 19 Apr 2026 12:36:44 -0700 Subject: [PATCH 09/22] Add back `Levenshtein.ratio` due to runtime impact --- poetry.lock | 194 +++++++++++++++++++++++++++++- pyproject.toml | 1 + sentieon_cli/scripts/indel2cnv.py | 51 ++++---- 3 files changed, 220 insertions(+), 26 deletions(-) diff --git a/poetry.lock b/poetry.lock index e7056ed..613fe92 100644 --- a/poetry.lock +++ b/poetry.lock @@ -459,6 +459,102 @@ files = [ {file = "iniconfig-2.3.0.tar.gz", hash = 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b/sentieon_cli/scripts/indel2cnv.py @@ -1,4 +1,5 @@ import collections +import Levenshtein import math import io import vcflib @@ -17,7 +18,7 @@ # When launched as `python indel2cnv.py ...` from an arbitrary CWD, # `sentieon_cli` is only importable if the package root is on sys.path. sys.path.insert(0, str(pathlib.Path(__file__).resolve().parents[2])) -from sentieon_cli._indel2cnv_utils import find_peaks, ratio # noqa: E402 +from sentieon_cli._indel2cnv_utils import find_peaks # noqa: E402 # Convert long INDELs (from assembly-based SV calls) into tandem CNV truth events. # Pipeline: find_repeats (detect period) -> match_ref (locate repeat on reference) @@ -100,13 +101,13 @@ def find_repeats(var_seq, thresh): step = 200 if length > 2000 else 100 start = step * 2 scan_limit = min(length//2+step*5, length-step*1) - dist = [ratio(var_seq[:n], var_seq[n:n*2], threshold=thresh) for n in range(start, scan_limit, step)] + dist = [Levenshtein.ratio(var_seq[:n], var_seq[n:n*2]) for n in range(start, scan_limit, step)] if not dist or max(dist) < thresh: return [len(var_seq)] peaks = find_peaks(dist, distance=start//step, height=0.95) if len(peaks) == 0: mid = length//2 - if ratio(var_seq[:mid], var_seq[mid:mid*2], threshold=thresh) > thresh: + if Levenshtein.ratio(var_seq[:mid], var_seq[mid:mid*2]) > thresh: peaks = [mid/step] else: peaks = [p+start//step for p in peaks] @@ -120,14 +121,14 @@ def find_repeats(var_seq, thresh): p_min, p_max = int(length/(cnt+0.5)), int(length/(cnt-0.5)) p_step = max(1, (p_max - p_min)//10) while True: - ratios = [max([ratio(var_seq[i*p:(i+1)*p], var_seq[(i+1)*p:(i+2)*p], threshold=thresh) for i in range(cnt-1)]) for p in range(p_min, p_max+1, p_step)] + ratios = [max([Levenshtein.ratio(var_seq[i*p:(i+1)*p], var_seq[(i+1)*p:(i+2)*p]) for i in range(cnt-1)]) for p in range(p_min, p_max+1, p_step)] best_ratio = max(ratios) best_period = p_min + (ratios.index(best_ratio))*p_step if p_step == 1: break p_min, p_max = best_period - 2 * p_step, best_period + 2 * p_step p_step = max(1, (p_max - p_min)//10) - ratio_orig = max([ratio(var_seq[i*period:(i+1)*period], var_seq[(i+1)*period:(i+2)*period], threshold=thresh) for i in range(cnt-1)]) + ratio_orig = max([Levenshtein.ratio(var_seq[i*period:(i+1)*period], var_seq[(i+1)*period:(i+2)*period]) for i in range(cnt-1)]) if best_ratio > ratio_orig: period = best_period periods = {period: max(best_ratio, ratio_orig)} @@ -138,7 +139,7 @@ def find_repeats(var_seq, thresh): break cur_cnt = int(round(length/cur_period)) if cur_cnt >= 2: - periods[cur_period] = max([ratio(var_seq[j*cur_period:(j+1)*cur_period], var_seq[(j+1)*cur_period:(j+2)*cur_period], threshold=thresh) for j in range(cur_cnt-1)]) + periods[cur_period] = max([Levenshtein.ratio(var_seq[j*cur_period:(j+1)*cur_period], var_seq[(j+1)*cur_period:(j+2)*cur_period]) for j in range(cur_cnt-1)]) i += 1 periods = {k: v for k, v in periods.items() if k >= MIN_SEQ_LEN} if len(periods) > 1: @@ -186,7 +187,7 @@ def mm2_match(seq, ref, chrom, pos, half_win, thresh): matched_ref_length = ref_stop - ref_start best_matched_ref = ref.get(chrom, ref_start, ref_stop) matched_seq = seq[seq_start:seq_stop] - best_ratio = ratio(best_matched_ref, matched_seq, threshold=thresh) + best_ratio = Levenshtein.ratio(best_matched_ref, matched_seq) if best_ratio < thresh: return None # Partial match extension: when mappy aligned only part of seq, the period boundary @@ -206,10 +207,10 @@ def mm2_match(seq, ref, chrom, pos, half_win, thresh): best_ext = 0 while True: for l in range(start_i, end_i, step): - r = ratio(best_matched_ref + ref_after[:l], seq[seq_start:]+seq[:l-seq_len_diff], threshold=thresh) - if r > thresh: + ratio = Levenshtein.ratio(best_matched_ref + ref_after[:l], seq[seq_start:]+seq[:l-seq_len_diff]) + if ratio > thresh: last_valid = l - last_valid_ratio = r + last_valid_ratio = ratio else: last_invalid = l break @@ -239,10 +240,10 @@ def mm2_match(seq, ref, chrom, pos, half_win, thresh): best_ext = 0 while True: for l in range(start_i, end_i, step): - r = ratio(ref_before[-l:] + best_matched_ref, seq[-l+seq_start:] + seq[:seq_stop], threshold=thresh) - if r > thresh: + ratio = Levenshtein.ratio(ref_before[-l:] + best_matched_ref, seq[-l+seq_start:] + seq[:seq_stop]) + if ratio > thresh: last_valid = l - last_valid_ratio = r + last_valid_ratio = ratio else: last_invalid = l break @@ -266,11 +267,11 @@ def mm2_match(seq, ref, chrom, pos, half_win, thresh): ref_stop_ext = ref_stop for _ in range(MAX_EXTEND_ITERS): ref_start_ext -= matched_ref_length - if ref_start_ext < 0 or ratio(ref.get(chrom, ref_start_ext, ref_start_ext+matched_ref_length), best_matched_ref, threshold=thresh) < thresh: + if ref_start_ext < 0 or Levenshtein.ratio(ref.get(chrom, ref_start_ext, ref_start_ext+matched_ref_length), best_matched_ref) < thresh: break for _ in range(MAX_EXTEND_ITERS): ref_stop_ext += matched_ref_length - if ratio(ref.get(chrom, ref_stop_ext-matched_ref_length, ref_stop_ext), best_matched_ref, threshold=thresh) < thresh: + if Levenshtein.ratio(ref.get(chrom, ref_stop_ext-matched_ref_length, ref_stop_ext), best_matched_ref) < thresh: break return (ref_start, ref_stop), (ref_start_ext, ref_stop_ext), (seq_start, seq_stop), best_ratio @@ -291,8 +292,8 @@ def local_match(v, ref, period, thresh): elif offset < 0: ref_after = ref_after[-offset:] alt_period_seq = alt_seq[:period] - ratios = {p: ratio(ref_after[:p] + ref_before[-(period-p):], alt_period_seq, threshold=0.8) for p in range(0, period, step)} - ratios[period] = ratio(ref_after, alt_period_seq, threshold=0.8) + ratios = {p: Levenshtein.ratio(ref_after[:p] + ref_before[-(period-p):], alt_period_seq) for p in range(0, period, step)} + ratios[period] = Levenshtein.ratio(ref_after, alt_period_seq) while True: best_pos = max(ratios, key=ratios.get) max_ratio = ratios[best_pos] @@ -305,7 +306,7 @@ def local_match(v, ref, period, thresh): p_hi = min(best_pos + step, period) for p in (p_lo, p_hi): if p not in ratios: - ratios[p] = ratio(ref_after[:p] + ref_before[-(period-p):], alt_period_seq, threshold=0.8) + ratios[p] = Levenshtein.ratio(ref_after[:p] + ref_before[-(period-p):], alt_period_seq) if max_ratio < thresh: return None ref_start = v.pos - (period - best_pos) @@ -320,11 +321,11 @@ def local_match(v, ref, period, thresh): ref_end_ext = ref_end for _ in range(MAX_EXTEND_ITERS): ref_start_ext -= period - if ref_start_ext < 0 or ratio(ref.get(v.chrom, ref_start_ext, ref_start_ext+period), ref_seq, threshold=thresh) < thresh: + if ref_start_ext < 0 or Levenshtein.ratio(ref.get(v.chrom, ref_start_ext, ref_start_ext+period), ref_seq) < thresh: break for _ in range(MAX_EXTEND_ITERS): ref_end_ext += period - if ratio(ref.get(v.chrom, ref_end_ext-period, ref_end_ext), ref_seq, threshold=thresh) < thresh: + if Levenshtein.ratio(ref.get(v.chrom, ref_end_ext-period, ref_end_ext), ref_seq) < thresh: break return (ref_start, ref_end), (ref_start_ext, ref_end_ext), (0, period), max_ratio @@ -354,11 +355,11 @@ def proc_variant(v, ref, thresh): if len(v.ref) > MIN_SEQ_LEN and len(v.ref) - len(a) > MIN_SEQ_LEN: v1 = copy_variant(v, alt=[a]) if len(a) > 1: - if a == v1.ref[:len(a)] or len(a) < 10 or ratio(a, v1.ref[:len(a)], threshold=thresh) > thresh: + if a == v1.ref[:len(a)] or len(a) < 10 or Levenshtein.ratio(a, v1.ref[:len(a)]) > thresh: v1.pos += len(a) - 1 v1.ref = v.ref[len(a):] v1.alt = [a[-1]] - elif ratio(a, v.ref[-len(a):], threshold=thresh) > thresh: + elif Levenshtein.ratio(a, v.ref[-len(a):]) > thresh: v1.ref = v.ref[:-len(a)] v1.alt = [v.ref[0]] v1.samples[0]['GT'] = '0/1' @@ -367,11 +368,11 @@ def proc_variant(v, ref, thresh): elif len(a) - len(v.ref) > MIN_SEQ_LEN: v1 = copy_variant(v) v1.samples[0]['GT'] = '0/1' - if len(v.ref) < 6 or ratio(v.alt[i][:len(v.ref)], v.ref, threshold=min(thresh, 1-5/len(v.ref))) > min(thresh, 1-5/len(v.ref)): + if len(v.ref) < 6 or Levenshtein.ratio(v.alt[i][:len(v.ref)], v.ref) > min(thresh, 1-5/len(v.ref)): v1.alt = [v1.alt[i][len(v.ref)-1:]] v1.pos += len(v.ref) - 1 v1.ref = v.ref[-1] - elif len(v.ref) < 6 or ratio(v.alt[i][-len(v.ref):], v.ref, threshold=min(thresh, 1-5/len(v.ref))) > min(thresh, 1-5/len(v.ref)): + elif len(v.ref) < 6 or Levenshtein.ratio(v.alt[i][-len(v.ref):], v.ref) > min(thresh, 1-5/len(v.ref)): v1.alt = [v1.alt[i][:-len(v.ref)]] v1.ref = v.ref[0] else: @@ -416,7 +417,7 @@ def proc_variant(v, ref, thresh): updated_periods = [] for p0 in periods[0]: p1s = [p1 for p1 in periods[1] if abs(p0/p1-1) <= 0.05] - if p1s and ratio(*(a[:min(p0, p1s[0])] for a in v.alt), threshold=1 - (1-thresh)/2) > 1 - (1-thresh)/2: + if p1s and Levenshtein.ratio(*(a[:min(p0, p1s[0])] for a in v.alt)) > 1 - (1-thresh)/2: updated_periods.append(min(p0, p1s[0])) if updated_periods: periods = [updated_periods] From ca9b800e0c82308483ac3fc3f9cb77f199a6e8e7 Mon Sep 17 00:00:00 2001 From: Don Freed Date: Sun, 19 Apr 2026 12:51:56 -0700 Subject: [PATCH 10/22] Automate release upload to PyPI --- .github/workflows/release.yml | 26 ++++++++++++++++++++++++-- 1 file changed, 24 insertions(+), 2 deletions(-) diff --git a/.github/workflows/release.yml b/.github/workflows/release.yml index 3b02b53..6a70ce0 100644 --- a/.github/workflows/release.yml +++ b/.github/workflows/release.yml @@ -26,7 +26,29 @@ jobs: sentieon-cli -h - name: build run: | - poetry build -f sdist - gh release upload ${{github.event.release.tag_name}} dist/*.tar.gz + poetry build + gh release upload ${{github.event.release.tag_name}} dist/*.tar.gz dist/*.whl env: GITHUB_TOKEN: ${{ github.TOKEN }} + - name: Upload distribution artifacts + uses: actions/upload-artifact@v4 + with: + name: dist + path: dist/* + + publish-pypi: + needs: build-release + runs-on: ubuntu-22.04 + environment: + name: pypi + url: https://pypi.org/p/sentieon_cli + permissions: + id-token: write + steps: + - name: Download distribution artifacts + uses: actions/download-artifact@v4 + with: + name: dist + path: dist + - name: Publish to PyPI + uses: 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Python library for VCF parsing and manipulation" +optional = false +python-versions = "!=3.0.*,!=3.1.*,!=3.2.*,!=3.3.*,!=3.4.*,!=3.5.*,>=2.7,>=3.6" +groups = ["main"] +files = [ + {file = "vcflib-1.0.1-py3-none-any.whl", hash = "sha256:26437875d441926b9fc6de2ce455c3516e6d6a4cce2e5ce638a94b05c803e705"}, + {file = "vcflib-1.0.1.tar.gz", hash = "sha256:0b814ea000c54c9885d63713c2a631a82f4bac9fba0f42a2c9c57fa8bed04759"}, +] + [[package]] name = "zipp" version = "3.23.1" @@ -1756,19 +2587,7 @@ enabler = ["pytest-enabler (>=2.2)"] test = ["big-O", "jaraco.functools", "jaraco.itertools", "jaraco.test", "more_itertools", "pytest (>=6,!=8.1.*)", "pytest-ignore-flaky"] type = ["pytest-mypy"] -[[package]] -name = "vcflib" -version = "1.0.1" -description = "An open-source Python library for VCF parsing and manipulation" -optional = false -python-versions = "!=3.0.*,!=3.1.*,!=3.2.*,!=3.3.*,!=3.4.*,!=3.5.*,>=2.7,>=3.6" -groups = ["main"] -files = [ - {file = "vcflib-1.0.1-py3-none-any.whl", hash = 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[published] + workflow_dispatch: + inputs: + build_tag: + description: "Build tag suffix (appended after the cli version)" + default: "0" + required: true + +permissions: + contents: read + +jobs: + build-push: + runs-on: ubuntu-22.04 + steps: + - uses: actions/checkout@v5 + - uses: actions/setup-python@v5 + with: + python-version: "3.14" + - name: Verify SENTIEON_VERSION repo variable + env: + SENTIEON_VERSION: ${{ vars.SENTIEON_VERSION }} + run: | + if [ -z "$SENTIEON_VERSION" ]; then + echo "::error::Repository variable SENTIEON_VERSION is not set." >&2 + exit 1 + fi + echo "Building against Sentieon $SENTIEON_VERSION" + - name: Resolve build metadata + id: meta + run: | + version=$(python -c 'import tomllib; print(tomllib.load(open("pyproject.toml","rb"))["project"]["version"])') + echo "version=$version" >> "$GITHUB_OUTPUT" + echo "build_date=$(date -u +%Y-%m-%dT%H:%M:%SZ)" >> "$GITHUB_OUTPUT" + - uses: docker/setup-buildx-action@v3 + - uses: docker/login-action@v3 + with: + username: ${{ secrets.DOCKERHUB_USERNAME }} + password: ${{ secrets.DOCKERHUB_TOKEN }} + - uses: docker/build-push-action@v6 + with: + context: . + platforms: linux/amd64 + push: true + tags: | + sentieon/sentieon-cli:${{ steps.meta.outputs.version }}-${{ inputs.build_tag || '0' }} + sentieon/sentieon-cli:latest + build-args: | + SENTIEON_VERSION=${{ vars.SENTIEON_VERSION }} + VCS_REF=${{ github.sha }} + BUILD_DATE=${{ steps.meta.outputs.build_date }} + cache-from: type=gha + cache-to: type=gha,mode=max From 5cc0015e3968e7bea733b18e97bb32cb95c0fb74 Mon Sep 17 00:00:00 2001 From: Don Freed Date: Wed, 22 Apr 2026 14:12:26 -0700 Subject: [PATCH 13/22] Add handling of difficult segdups --- sentieon_cli/command_strings.py | 9 ++-- sentieon_cli/sentieon_pangenome.py | 70 ++++++++++++++++++++++++++++++ 2 files changed, 74 insertions(+), 5 deletions(-) diff --git a/sentieon_cli/command_strings.py b/sentieon_cli/command_strings.py index 3e49970..e48fae4 100644 --- a/sentieon_cli/command_strings.py +++ b/sentieon_cli/command_strings.py @@ -1236,7 +1236,7 @@ def cmd_segdup_caller( sr_alignments: pathlib.Path, reference: pathlib.Path, sr_bundle: pathlib.Path, - genes="CFH,CFHR3,CYP11B1,CYP2D6,GBA,NCF1,PMS2,SMN1,STRC", + genes: Optional[str] = None, ) -> Pipeline: cmd = [ "segdup-caller", @@ -1246,11 +1246,10 @@ def cmd_segdup_caller( str(reference), "--sr_model", str(sr_bundle), - "--genes", - genes, - "--outdir", - str(out_segdup), ] + if genes: + cmd.extend(["--genes", genes]) + cmd.extend(["--outdir", str(out_segdup)]) return Pipeline(Command(*cmd)) diff --git a/sentieon_cli/sentieon_pangenome.py b/sentieon_cli/sentieon_pangenome.py index 89bbe36..ad58a10 100644 --- a/sentieon_cli/sentieon_pangenome.py +++ b/sentieon_cli/sentieon_pangenome.py @@ -65,6 +65,10 @@ "samtools": packaging.version.Version("1.16"), } +SEGDUP_MIN_VERSION = { + "segdup-caller": None, +} + logger = get_logger(__name__) @@ -128,6 +132,16 @@ class SentieonPangenome(BasePangenome): "help": "Skip multiQC report generation", "action": "store_true", }, + "segdup_caller": { + "nargs": "*", + "help": ( + "Call variants in difficult segmental duplications with " + "segdup-caller. Supply the flag with no arguments to run " + "the caller's default gene set. Supply a comma-separated " + "list of gene names (e.g. 'CFH,CYP2D6,SMN1') to restrict " + "calling to those genes." + ), + }, # Hidden arguments "skip_contig_checks": { "help": argparse.SUPPRESS, @@ -166,6 +180,7 @@ def __init__(self) -> None: self.pangenome_contig_prefix = "GRCh38#0#" self.skip_metrics = False self.skip_multiqc = False + self.segdup_caller: Optional[List[str]] = None self.skip_contig_checks: bool = False self.skip_pangenome_name_checks: bool = False self.skip_pop_vcf_id_check: bool = False @@ -224,6 +239,8 @@ def validate(self) -> None: if self.r1_fastq: self.validate_bwa_index() + self.validate_segdup() + if not self.skip_version_check: for cmd, min_version in SENT_PANGENOME_MIN_VERSIONS.items(): if not check_version(cmd, min_version): @@ -291,6 +308,22 @@ def validate(self) -> None: ) sys.exit(2) + def validate_segdup(self) -> None: + if self.segdup_caller is None: + return + + if len(self.sample_input) > 1: + self.logger.error( + "`--segdup_caller` accepts only a single `--sample_input` " + "BAM/CRAM file." + ) + sys.exit(2) + + if not self.skip_version_check: + for cmd, min_version in SEGDUP_MIN_VERSION.items(): + if not check_version(cmd, min_version): + sys.exit(2) + def validate_bundle(self) -> None: if not self.pop_vcf: self.logger.error("pop_vcf is required") @@ -566,6 +599,17 @@ def build_first_dag(self) -> DAG: dnascope_bams.append(mm2_bam) cnv_input_bams = list(self.sample_input) + # SegDup calling on the short-read alignment + if self.segdup_caller is not None: + out_segdup = pathlib.Path( + str(self.output_vcf).replace(".vcf.gz", "_segdups") + ) + genes = ",".join(self.segdup_caller) or None + segdup_job = self.build_segdup_job( + out_segdup, cnv_input_bams[0], genes + ) + dag.add_job(segdup_job, cnvscope_deps) + # DNAscope calling with bwa and mm2 input sv_vcf = None if self.call_svs: @@ -974,6 +1018,32 @@ def build_dnamodelapply_job(self, in_vcf) -> Job: self.cores, ) + def build_segdup_job( + self, + out_segdup: pathlib.Path, + sr_alignment: pathlib.Path, + genes: Optional[str], + ) -> Job: + """Call variants in difficult SegDups""" + if not self.reference: + self.logger.error("reference is required") + sys.exit(2) + if not self.model_bundle: + self.logger.error("model_bundle is required") + sys.exit(2) + + return Job( + cmds.cmd_segdup_caller( + out_segdup, + sr_alignment, + reference=self.reference, + sr_bundle=self.model_bundle, + genes=genes, + ), + "segdup-caller", + self.cores, + ) + def _add_cnv_jobs( self, dag: DAG, From 1adc5fe12ecfcc0cec407978a1d9e4570e35ff28 Mon Sep 17 00:00:00 2001 From: Don Freed Date: Thu, 23 Apr 2026 08:06:14 -0700 Subject: [PATCH 14/22] Add STR calling to the pangenome pipeline --- sentieon_cli/sentieon_pangenome.py | 87 +++++++++++++++++++++++++++++- 1 file changed, 86 insertions(+), 1 deletion(-) diff --git a/sentieon_cli/sentieon_pangenome.py b/sentieon_cli/sentieon_pangenome.py index ad58a10..fb47964 100644 --- a/sentieon_cli/sentieon_pangenome.py +++ b/sentieon_cli/sentieon_pangenome.py @@ -16,7 +16,7 @@ from . import command_strings as cmds from .archive import ar_load -from .base_pangenome import BasePangenome +from .base_pangenome import BasePangenome, SampleSex from .dag import DAG from .driver import ( AlignmentStat, @@ -69,6 +69,10 @@ "segdup-caller": None, } +EXPANSION_MIN_VERSION = { + "ExpansionHunter": None, +} + logger = get_logger(__name__) @@ -142,6 +146,13 @@ class SentieonPangenome(BasePangenome): "calling to those genes." ), }, + "expansion_catalog": { + "help": ( + "An ExpansionHunter variant catalog. Required for short " + "tandem repeat expansion calling." + ), + "type": path_arg(exists=True, is_file=True), + }, # Hidden arguments "skip_contig_checks": { "help": argparse.SUPPRESS, @@ -181,6 +192,7 @@ def __init__(self) -> None: self.skip_metrics = False self.skip_multiqc = False self.segdup_caller: Optional[List[str]] = None + self.expansion_catalog: Optional[pathlib.Path] = None self.skip_contig_checks: bool = False self.skip_pangenome_name_checks: bool = False self.skip_pop_vcf_id_check: bool = False @@ -211,6 +223,12 @@ def main(self, args: argparse.Namespace) -> None: executor = self.run(dag) self.check_execution(dag, executor) + if self.expansion_catalog: + self.get_sex(self.ploidy_json) + dag = self.build_second_dag() + executor = self.run(dag) + self.check_execution(dag, executor) + if not self.retain_tmpdir: shutil.rmtree(tmp_dir_str) @@ -240,6 +258,7 @@ def validate(self) -> None: self.validate_bwa_index() self.validate_segdup() + self.validate_expansion() if not self.skip_version_check: for cmd, min_version in SENT_PANGENOME_MIN_VERSIONS.items(): @@ -324,6 +343,22 @@ def validate_segdup(self) -> None: if not check_version(cmd, min_version): sys.exit(2) + def validate_expansion(self) -> None: + if self.expansion_catalog is None: + return + + if len(self.sample_input) > 1: + self.logger.error( + "`--expansion_catalog` accepts only a single `--sample_input` " + "BAM/CRAM file." + ) + sys.exit(2) + + if not self.skip_version_check: + for cmd, min_version in EXPANSION_MIN_VERSION.items(): + if not check_version(cmd, min_version): + sys.exit(2) + def validate_bundle(self) -> None: if not self.pop_vcf: self.logger.error("pop_vcf is required") @@ -599,6 +634,9 @@ def build_first_dag(self) -> DAG: dnascope_bams.append(mm2_bam) cnv_input_bams = list(self.sample_input) + # Stash the short-read alignment for the second DAG + self.sr_alignment = cnv_input_bams[0] + # SegDup calling on the short-read alignment if self.segdup_caller is not None: out_segdup = pathlib.Path( @@ -610,6 +648,13 @@ def build_first_dag(self) -> DAG: ) dag.add_job(segdup_job, cnvscope_deps) + # Estimate ploidy when needed for sex-aware downstream tools + if self.expansion_catalog: + ploidy_job = self.build_ploidy_job( + self.ploidy_json, [cnv_input_bams[0]] + ) + dag.add_job(ploidy_job, cnvscope_deps) + # DNAscope calling with bwa and mm2 input sv_vcf = None if self.call_svs: @@ -1044,6 +1089,46 @@ def build_segdup_job( self.cores, ) + def build_second_dag(self) -> DAG: + """Build the second DAG for sex-aware downstream tools""" + self.logger.info("Building the second pangenome DAG") + dag = DAG() + + if self.expansion_catalog and self.sr_alignment: + out_expansions = pathlib.Path( + str(self.output_vcf).replace(".vcf.gz", "_expansion") + ) + expansion_job = self.build_expansion_job( + out_expansions, self.sr_alignment, self.expansion_catalog + ) + dag.add_job(expansion_job) + + return dag + + def build_expansion_job( + self, + out_expansions: pathlib.Path, + sr_alignment: pathlib.Path, + expansion_catalog: pathlib.Path, + ) -> Job: + """Identify repeat expansions""" + if not self.reference: + self.logger.error("reference is required") + sys.exit(2) + + return Job( + cmds.cmd_expansion_hunter( + out_expansions, + sr_alignment, + reference=self.reference, + variant_catalog=expansion_catalog, + sex="male" if self.sample_sex == SampleSex.MALE else "female", + threads=self.cores, + ), + "expansion-hunter", + self.cores, + ) + def _add_cnv_jobs( self, dag: DAG, From f5f35bdd6fcb6f6b895322909b481cf3a0650029 Mon Sep 17 00:00:00 2001 From: Don Freed Date: Tue, 28 Apr 2026 09:54:44 -0700 Subject: [PATCH 15/22] Enable SV calling without a `cnv.model` file --- sentieon_cli/sentieon_pangenome.py | 28 ++++++++++---------- tests/unit/test_sentieon_pangenome.py | 37 +++++++++++++++++++++++++++ 2 files changed, 52 insertions(+), 13 deletions(-) diff --git a/sentieon_cli/sentieon_pangenome.py b/sentieon_cli/sentieon_pangenome.py index fb47964..46ead5d 100644 --- a/sentieon_cli/sentieon_pangenome.py +++ b/sentieon_cli/sentieon_pangenome.py @@ -417,12 +417,13 @@ def validate_bundle(self) -> None: ) sys.exit(2) - if self.call_svs and "cnv.model" not in bundle_members: - self.logger.error( - "The model bundle does not contain a 'cnv.model' file " - "required for CNV calling with `--call_svs`." + self.has_cnv_model = "cnv.model" in bundle_members + if self.call_svs and not self.has_cnv_model: + self.logger.warning( + "The model bundle does not contain a 'cnv.model' file. " + "CNV calling with CNVscope will be skipped; SV calling with " + "PangenomeSV will still run." ) - sys.exit(2) if not self.skip_pop_vcf_id_check and not self.dry_run: pop_vcf_id = vcf_id(self.pop_vcf) @@ -674,13 +675,14 @@ def build_first_dag(self) -> DAG: gfa_file=sample_gfa, ) dag.add_job(dnascope_job, dnascope_dependencies) - self._add_cnv_jobs( - dag, - sv_vcf, - cnv_input_bams, - cnvscope_deps, - dnascope_job, - ) + if self.has_cnv_model: + self._add_cnv_jobs( + dag, + sv_vcf, + cnv_input_bams, + cnvscope_deps, + dnascope_job, + ) return dag apply_dependencies = set() @@ -716,7 +718,7 @@ def build_first_dag(self) -> DAG: apply_job = self.build_dnamodelapply_job(transfer_vcf) dag.add_job(apply_job, apply_dependencies) - if self.call_svs and sv_vcf: + if self.call_svs and sv_vcf and self.has_cnv_model: self._add_cnv_jobs( dag, sv_vcf, diff --git a/tests/unit/test_sentieon_pangenome.py b/tests/unit/test_sentieon_pangenome.py index b412389..8ecb709 100644 --- a/tests/unit/test_sentieon_pangenome.py +++ b/tests/unit/test_sentieon_pangenome.py @@ -86,6 +86,7 @@ def create_pipeline(self): pipeline.bam_readgroups = [{"ID": "rg1", "SM": "sample1"}] pipeline.fastq_readgroup = {} pipeline.tech = "Illumina" + pipeline.has_cnv_model = True pipeline.pcr_free = False return pipeline @@ -272,6 +273,42 @@ def test_cnv_jobs_with_skip_small_variants(self): assert "indel2cnv" in job_names assert "combine-cnv" in job_names + def test_call_svs_without_cnv_model(self): + """SVs run but CNV jobs are skipped when bundle lacks cnv.model""" + pipeline = self.create_pipeline() + pipeline.call_svs = True + pipeline.has_cnv_model = False + dag = pipeline.build_first_dag() + + job_names, all_jobs = self._get_all_job_names(dag) + + # PangenomeSV still runs + assert "dnascope-raw" in job_names + dnascope_job = next(j for j in all_jobs if j.name == "dnascope-raw") + cmd_str = str(dnascope_job.shell) + assert "--algo PangenomeSV" in cmd_str + + # CNV jobs are skipped + assert "cnvscope" not in job_names + assert "cnv-model-apply" not in job_names + assert "indel2cnv" not in job_names + assert "combine-cnv" not in job_names + + def test_call_svs_without_cnv_model_skip_small_variants(self): + """SV-only mode: SVs run but CNV jobs are skipped without cnv.model""" + pipeline = self.create_pipeline() + pipeline.skip_small_variants = True + pipeline.call_svs = True + pipeline.has_cnv_model = False + dag = pipeline.build_first_dag() + + job_names, _ = self._get_all_job_names(dag) + assert "dnascope-raw" in job_names + assert "cnvscope" not in job_names + assert "cnv-model-apply" not in job_names + assert "indel2cnv" not in job_names + assert "combine-cnv" not in job_names + def test_cnvscope_command(self): """Test CNVscope driver command has correct algo and model""" pipeline = self.create_pipeline() From 4b3d705513db98141b972d05190b685e45b976f6 Mon Sep 17 00:00:00 2001 From: Don Freed Date: Wed, 29 Apr 2026 16:48:38 -0700 Subject: [PATCH 16/22] Add HLA/KIR calling with t1k --- sentieon_cli/sentieon_pangenome.py | 164 +++++++++++++++++++++++++++++ 1 file changed, 164 insertions(+) diff --git a/sentieon_cli/sentieon_pangenome.py b/sentieon_cli/sentieon_pangenome.py index 46ead5d..fc5ecc6 100644 --- a/sentieon_cli/sentieon_pangenome.py +++ b/sentieon_cli/sentieon_pangenome.py @@ -34,6 +34,7 @@ MeanQualityByCycle, PangenomeSV, QualDistribution, + ReadWriter, WgsMetricsAlgo, ) from .job import Job @@ -73,6 +74,13 @@ "ExpansionHunter": None, } +T1K_MIN_VERSION = { + "run-t1k": None, +} + +DEFAULT_T1K_HLA_LOCUS = "chr6:28510020-33480577" +DEFAULT_T1K_KIR_LOCUS = "chr19:53100000-55800000" + logger = get_logger(__name__) @@ -153,6 +161,50 @@ class SentieonPangenome(BasePangenome): ), "type": path_arg(exists=True, is_file=True), }, + "t1k_hla_seq": { + "help": ( + "The DNA HLA seq FASTA file for T1K. Required for HLA " + "calling." + ), + "type": path_arg(exists=True, is_file=True), + }, + "t1k_hla_coord": { + "help": ( + "The DNA HLA coord FASTA file for T1K. Required for HLA " + "calling." + ), + "type": path_arg(exists=True, is_file=True), + }, + "t1k_hla_locus": { + "default": DEFAULT_T1K_HLA_LOCUS, + "help": ( + "Reference interval covering the HLA locus. Reads " + "overlapping this region are extracted before being " + f"passed to T1K (default: {DEFAULT_T1K_HLA_LOCUS})." + ), + }, + "t1k_kir_seq": { + "help": ( + "The DNA KIR seq FASTA file for T1K. Required for KIR " + "calling." + ), + "type": path_arg(exists=True, is_file=True), + }, + "t1k_kir_coord": { + "help": ( + "The DNA KIR coord FASTA file for T1K. Required for KIR " + "calling." + ), + "type": path_arg(exists=True, is_file=True), + }, + "t1k_kir_locus": { + "default": DEFAULT_T1K_KIR_LOCUS, + "help": ( + "Reference interval covering the KIR locus. Reads " + "overlapping this region are extracted before being " + f"passed to T1K (default: {DEFAULT_T1K_KIR_LOCUS})." + ), + }, # Hidden arguments "skip_contig_checks": { "help": argparse.SUPPRESS, @@ -193,6 +245,12 @@ def __init__(self) -> None: self.skip_multiqc = False self.segdup_caller: Optional[List[str]] = None self.expansion_catalog: Optional[pathlib.Path] = None + self.t1k_hla_seq: Optional[pathlib.Path] = None + self.t1k_hla_coord: Optional[pathlib.Path] = None + self.t1k_hla_locus: str = DEFAULT_T1K_HLA_LOCUS + self.t1k_kir_seq: Optional[pathlib.Path] = None + self.t1k_kir_coord: Optional[pathlib.Path] = None + self.t1k_kir_locus: str = DEFAULT_T1K_KIR_LOCUS self.skip_contig_checks: bool = False self.skip_pangenome_name_checks: bool = False self.skip_pop_vcf_id_check: bool = False @@ -259,6 +317,7 @@ def validate(self) -> None: self.validate_segdup() self.validate_expansion() + self.validate_t1k() if not self.skip_version_check: for cmd, min_version in SENT_PANGENOME_MIN_VERSIONS.items(): @@ -359,6 +418,35 @@ def validate_expansion(self) -> None: if not check_version(cmd, min_version): sys.exit(2) + def validate_t1k(self) -> None: + hla_requested = self.t1k_hla_seq is not None or ( + self.t1k_hla_coord is not None + ) + if hla_requested and not (self.t1k_hla_seq and self.t1k_hla_coord): + self.logger.error( + "For HLA calling, both `--t1k_hla_seq` and `--t1k_hla_coord` " + "must be supplied." + ) + sys.exit(2) + + kir_requested = self.t1k_kir_seq is not None or ( + self.t1k_kir_coord is not None + ) + if kir_requested and not (self.t1k_kir_seq and self.t1k_kir_coord): + self.logger.error( + "For KIR calling, both `--t1k_kir_seq` and `--t1k_kir_coord` " + "must be supplied." + ) + sys.exit(2) + + if not (hla_requested or kir_requested): + return + + if not self.skip_version_check: + for cmd, min_version in T1K_MIN_VERSION.items(): + if not check_version(cmd, min_version): + sys.exit(2) + def validate_bundle(self) -> None: if not self.pop_vcf: self.logger.error("pop_vcf is required") @@ -656,6 +744,38 @@ def build_first_dag(self) -> DAG: ) dag.add_job(ploidy_job, cnvscope_deps) + # T1K HLA/KIR calling + if self.t1k_hla_seq and self.t1k_hla_coord: + out_hla = pathlib.Path( + str(self.output_vcf).replace(".vcf.gz", "_hla") + ) + extract_job, t1k_job = self.build_t1k_jobs( + out_hla, + cnv_input_bams, + self.t1k_hla_seq, + self.t1k_hla_coord, + self.t1k_hla_locus, + "hla-wgs", + tag="hla", + ) + dag.add_job(extract_job, cnvscope_deps) + dag.add_job(t1k_job, {extract_job}) + if self.t1k_kir_seq and self.t1k_kir_coord: + out_kir = pathlib.Path( + str(self.output_vcf).replace(".vcf.gz", "_kir") + ) + extract_job, t1k_job = self.build_t1k_jobs( + out_kir, + cnv_input_bams, + self.t1k_kir_seq, + self.t1k_kir_coord, + self.t1k_kir_locus, + "kir-wgs", + tag="kir", + ) + dag.add_job(extract_job, cnvscope_deps) + dag.add_job(t1k_job, {extract_job}) + # DNAscope calling with bwa and mm2 input sv_vcf = None if self.call_svs: @@ -1107,6 +1227,50 @@ def build_second_dag(self) -> DAG: return dag + def build_t1k_jobs( + self, + out_basename: pathlib.Path, + sr_alignments: List[pathlib.Path], + gene_seq: pathlib.Path, + gene_coord: pathlib.Path, + locus: str, + preset: str, + tag: str, + ) -> Tuple[Job, Job]: + """Extract reads at the T1K locus and genotype them with T1K""" + if not self.reference: + self.logger.error("reference is required") + sys.exit(2) + + # Extract reads overlapping the locus to a BAM file with ReadWriter + extracted_bam = self.tmp_dir.joinpath(f"sample_{tag}.bam") + driver = Driver( + reference=self.reference, + thread_count=self.cores, + input=sr_alignments, + interval=locus, + ) + driver.add_algo(ReadWriter(extracted_bam)) + extract_job = Job( + Pipeline(Command(*driver.build_cmd())), + f"t1k-{tag}-extract", + self.cores, + ) + + t1k_job = Job( + cmds.cmd_t1k( + out_basename, + extracted_bam, + gene_seq=gene_seq, + gene_coord=gene_coord, + preset=preset, + threads=self.cores, + ), + f"t1k-{tag}", + self.cores, + ) + return (extract_job, t1k_job) + def build_expansion_job( self, out_expansions: pathlib.Path, From 6713db78e12d117ba807cb45f8842d08a9c646b4 Mon Sep 17 00:00:00 2001 From: Don Freed Date: Wed, 29 Apr 2026 18:43:34 -0700 Subject: [PATCH 17/22] Add new external tools to the Dockerfile --- Dockerfile | 71 +++++++++++++++++++++++++++++++++++++++++++++++++----- 1 file changed, 65 insertions(+), 6 deletions(-) diff --git a/Dockerfile b/Dockerfile index 7cfe398..71a77f3 100644 --- a/Dockerfile +++ b/Dockerfile @@ -19,6 +19,16 @@ ARG VG_SHA256=d5752977237e801d971f0d044f43fde4f3005da35f0451b4f452d1c1c9b8414b ARG KMC_VERSION=3.2.4 ARG KMC_RELEASE_TAG=v3.2.4-pipe2 ARG KMC_SHA256=d8bdf8edcd0577dba32e86e8f194b2eb04eb168c75a3e6d46721d5bef515ac96 +ARG EXPANSIONHUNTER_VERSION=5.0.0 +ARG EXPANSIONHUNTER_SHA256=ebf3ec0ace6e6e3bbce12c26463da5d9f8e16374eff1ad10f0f1a9123050fa86 +# T1K and segdup-caller are built from git; pin the tag and verify the +# resolved commit SHA after checkout. +ARG T1K_VERSION=1.0.9 +ARG T1K_GIT_TAG=v1.0.9 +ARG T1K_COMMIT=9376b555c1d8d2f8ca357c2656f49f450462dbc3 +ARG SEGDUP_CALLER_VERSION=0.5.0 +ARG SEGDUP_CALLER_GIT_TAG=v0.5.0 +ARG SEGDUP_CALLER_COMMIT=cc8ddfb7d8a18ff254c5126ec879e8a18ee28389 # Pinned Poetry toolchain; should match the version used to generate poetry.lock. ARG POETRY_VERSION=2.3.4 ARG POETRY_PLUGIN_EXPORT_VERSION=1.9.0 @@ -27,7 +37,7 @@ RUN test -n "$SENTIEON_VERSION" # Install all build dependencies for the downloader stage in one pass. RUN apt-get update && apt-get install -y --no-install-recommends \ - curl ca-certificates bzip2 autoconf automake libtool make gcc perl \ + curl ca-certificates bzip2 autoconf automake libtool make gcc g++ perl \ nasm git python3 python3-venv zlib1g-dev libbz2-dev liblzma-dev \ libcurl4-gnutls-dev libssl-dev libncurses5-dev libdeflate-dev \ libperl-dev libgsl0-dev && \ @@ -94,6 +104,31 @@ RUN mkdir -p /opt/kmc-${KMC_VERSION} && \ /usr/local/bin/kmc_tools-${KMC_VERSION} \ /usr/local/bin/kmc_dump-${KMC_VERSION} +# Install ExpansionHunter +RUN mkdir -p /tmp/eh && \ + curl -fL -o /tmp/eh.tar.gz "https://github.com/Illumina/ExpansionHunter/releases/download/v${EXPANSIONHUNTER_VERSION}/ExpansionHunter-v${EXPANSIONHUNTER_VERSION}-linux_x86_64.tar.gz" && \ + echo "${EXPANSIONHUNTER_SHA256} /tmp/eh.tar.gz" | sha256sum -c - && \ + tar -C /tmp/eh --strip-components=1 -zxf /tmp/eh.tar.gz && \ + cp /tmp/eh/bin/ExpansionHunter /usr/local/bin/ExpansionHunter-${EXPANSIONHUNTER_VERSION} && \ + chmod +x /usr/local/bin/ExpansionHunter-${EXPANSIONHUNTER_VERSION} && \ + rm -rf /tmp/eh /tmp/eh.tar.gz + +# Build T1K from source. +RUN git clone --branch ${T1K_GIT_TAG} https://github.com/mourisl/T1K.git /tmp/t1k-src && \ + cd /tmp/t1k-src && \ + test "$(git rev-parse HEAD)" = "${T1K_COMMIT}" && \ + make -j"$(nproc)" && \ + mkdir -p /opt/t1k-${T1K_VERSION} && \ + cp run-t1k bam-extractor fastq-extractor genotyper analyzer \ + /opt/t1k-${T1K_VERSION}/ && \ + rm -rf /tmp/t1k-src + +# Clone segdup-caller. The actual `pip install` happens in the +# python-builder stage so the venv lives in a clean image. +RUN git clone --branch ${SEGDUP_CALLER_GIT_TAG} https://github.com/Sentieon/segdup-caller.git /opt/segdup-caller-src && \ + cd /opt/segdup-caller-src && \ + test "$(git rev-parse HEAD)" = "${SEGDUP_CALLER_COMMIT}" + # Download the Sentieon software RUN mkdir -p /opt/sentieon/ && \ curl -fL "https://s3.amazonaws.com/sentieon-release/software/sentieon-genomics-${SENTIEON_VERSION}.tar.gz" | \ @@ -124,12 +159,16 @@ FROM debian:13.4-slim AS python-builder ARG SENTIEON_VERSION COPY --from=downloader /opt/sentieon-cli/dist /opt/sentieon-cli/dist +COPY --from=downloader /opt/segdup-caller-src /opt/segdup-caller-src +# Build the sentieon-cli venv and a separate venv for segdup-caller. +# segdup-caller pulls in whatshap/pysam/scipy/pandas which would otherwise +# bloat (and risk version-conflicting with) the sentieon-cli env. # Remove the kaleido and pyarrow packages to reduce image size. RUN apt-get update && \ apt-get install -y --no-install-recommends \ python3 python3-venv python3-dev \ - gcc g++ make zlib1g-dev && \ + gcc g++ make zlib1g-dev libbz2-dev liblzma-dev libcurl4-gnutls-dev && \ rm -rf /var/lib/apt/lists/* && \ python3 -m venv /opt/sentieon-cli-venv && \ /opt/sentieon-cli-venv/bin/pip install --no-cache-dir \ @@ -138,7 +177,12 @@ RUN apt-get update && \ /opt/sentieon-cli/dist/*.whl && \ /opt/sentieon-cli-venv/bin/pip uninstall -y kaleido pyarrow && \ /opt/sentieon-cli-venv/bin/pip uninstall -y pip setuptools wheel && \ - find /opt/sentieon-cli-venv -depth -type d -name __pycache__ -exec rm -rf {} + + python3 -m venv /opt/segdup-caller-venv && \ + /opt/segdup-caller-venv/bin/pip install --no-cache-dir \ + /opt/segdup-caller-src && \ + /opt/segdup-caller-venv/bin/pip uninstall -y pip setuptools wheel && \ + find /opt/sentieon-cli-venv /opt/segdup-caller-venv \ + -depth -type d -name __pycache__ -exec rm -rf {} + # Build the container FROM debian:13.4-slim @@ -147,6 +191,8 @@ ARG BEDTOOLS_VERSION=2.30.0 ARG MOSDEPTH_VERSION=0.3.9 ARG VG_VERSION=1.73.0 ARG KMC_VERSION=3.2.4 +ARG EXPANSIONHUNTER_VERSION=5.0.0 +ARG T1K_VERSION=1.0.9 ARG VCS_REF ARG BUILD_DATE @@ -175,10 +221,14 @@ COPY --from=downloader /usr/local/bin/vg-${VG_VERSION} /usr/local/bin/vg-${VG_VE COPY --from=downloader /usr/local/bin/kmc-${KMC_VERSION} /usr/local/bin/kmc-${KMC_VERSION} COPY --from=downloader /usr/local/bin/kmc_tools-${KMC_VERSION} /usr/local/bin/kmc_tools-${KMC_VERSION} COPY --from=downloader /usr/local/bin/kmc_dump-${KMC_VERSION} /usr/local/bin/kmc_dump-${KMC_VERSION} +COPY --from=downloader /usr/local/bin/ExpansionHunter-${EXPANSIONHUNTER_VERSION} /usr/local/bin/ExpansionHunter-${EXPANSIONHUNTER_VERSION} +COPY --from=downloader /opt/t1k-${T1K_VERSION} /opt/t1k-${T1K_VERSION} COPY --from=downloader /opt/sentieon-cli/dist /opt/sentieon-cli/dist # Create symlinks for libisal (the .so lives on the default linker path in # /usr/lib) and for the unversioned names of the third-party tools. +# `run-t1k` resolves its sibling binaries via abs_path($0), so symlinking +# it from /usr/local/bin keeps the binaries discoverable in /opt/t1k-*/. RUN cd /usr/lib && \ ln -s libisal.so.2.0.30 libisal.so.2 && \ ln -s libisal.so.2 libisal.so && \ @@ -188,13 +238,16 @@ RUN cd /usr/lib && \ ln -s vg-${VG_VERSION} vg && \ ln -s kmc-${KMC_VERSION} kmc && \ ln -s kmc_tools-${KMC_VERSION} kmc_tools && \ - ln -s kmc_dump-${KMC_VERSION} kmc_dump + ln -s kmc_dump-${KMC_VERSION} kmc_dump && \ + ln -s ExpansionHunter-${EXPANSIONHUNTER_VERSION} ExpansionHunter && \ + ln -s /opt/t1k-${T1K_VERSION}/run-t1k run-t1k # Install runtime dependencies. RUN apt-get update && \ apt-get install -y --no-install-recommends \ libjemalloc2 procps libdeflate0 libbz2-1.0 liblzma5 \ libcurl3-gnutls libssl3 libperl5.40 libgsl28 libncurses6 \ + libstdc++6 perl \ curl python3 && \ rm -rf /var/lib/apt/lists/* @@ -205,9 +258,12 @@ ENV LD_PRELOAD=/usr/lib/x86_64-linux-gnu/libjemalloc.so.2 # A default jemalloc configuration that should work well for most use-cases, see http://jemalloc.net/jemalloc.3.html ENV MALLOC_CONF=metadata_thp:auto,background_thread:true,dirty_decay_ms:30000,muzzy_decay_ms:30000 -# Copy the pre-built venv from the python-builder stage. No compilers -# are installed in this stage. +# Copy the pre-built venvs from the python-builder stage. No compilers +# are installed in this stage. The segdup-caller venv stays isolated; +# only its CLI entry point is exposed on PATH. COPY --from=python-builder /opt/sentieon-cli-venv /opt/sentieon-cli-venv +COPY --from=python-builder /opt/segdup-caller-venv /opt/segdup-caller-venv +RUN ln -s /opt/segdup-caller-venv/bin/segdup-caller /usr/local/bin/segdup-caller ENV VIRTUAL_ENV=/opt/sentieon-cli-venv ENV PATH=/opt/sentieon-cli-venv/bin:$PATH @@ -226,4 +282,7 @@ RUN sentieon driver --help && \ mosdepth -h && \ vg version && \ kmc --help && \ + ExpansionHunter --help && \ + perl -c "$(command -v run-t1k)" && \ + segdup-caller --version && \ sentieon-cli -h From 6e21d7e4ec89fe045cbf5077e2df8e9b54cdf6b9 Mon Sep 17 00:00:00 2001 From: Don Freed Date: Thu, 30 Apr 2026 22:03:29 -0700 Subject: [PATCH 18/22] Update segdup-caller command --- sentieon_cli/command_strings.py | 6 ++++ sentieon_cli/sentieon_pangenome.py | 47 +++++++++++++++++++++--------- 2 files changed, 39 insertions(+), 14 deletions(-) diff --git a/sentieon_cli/command_strings.py b/sentieon_cli/command_strings.py index e48fae4..b48936c 100644 --- a/sentieon_cli/command_strings.py +++ b/sentieon_cli/command_strings.py @@ -1236,6 +1236,8 @@ def cmd_segdup_caller( sr_alignments: pathlib.Path, reference: pathlib.Path, sr_bundle: pathlib.Path, + input_vcf: Optional[pathlib.Path] = None, + sex: Optional[str] = None, genes: Optional[str] = None, ) -> Pipeline: cmd = [ @@ -1247,6 +1249,10 @@ def cmd_segdup_caller( "--sr_model", str(sr_bundle), ] + if input_vcf is not None: + cmd.extend(["--input_vcf", str(input_vcf)]) + if sex is not None: + cmd.extend(["--sex", sex]) if genes: cmd.extend(["--genes", genes]) cmd.extend(["--outdir", str(out_segdup)]) diff --git a/sentieon_cli/sentieon_pangenome.py b/sentieon_cli/sentieon_pangenome.py index fc5ecc6..53d0bb5 100644 --- a/sentieon_cli/sentieon_pangenome.py +++ b/sentieon_cli/sentieon_pangenome.py @@ -67,7 +67,7 @@ } SEGDUP_MIN_VERSION = { - "segdup-caller": None, + "segdup-caller": packaging.version.Version("0.5.1"), } EXPANSION_MIN_VERSION = { @@ -281,7 +281,7 @@ def main(self, args: argparse.Namespace) -> None: executor = self.run(dag) self.check_execution(dag, executor) - if self.expansion_catalog: + if self.expansion_catalog or self.segdup_caller is not None: self.get_sex(self.ploidy_json) dag = self.build_second_dag() executor = self.run(dag) @@ -397,6 +397,14 @@ def validate_segdup(self) -> None: ) sys.exit(2) + if self.skip_small_variants: + self.logger.error( + "`--segdup_caller` requires the small-variant VCF as " + "`--input_vcf` and cannot be combined with " + "`--skip_small_variants`." + ) + sys.exit(2) + if not self.skip_version_check: for cmd, min_version in SEGDUP_MIN_VERSION.items(): if not check_version(cmd, min_version): @@ -726,19 +734,8 @@ def build_first_dag(self) -> DAG: # Stash the short-read alignment for the second DAG self.sr_alignment = cnv_input_bams[0] - # SegDup calling on the short-read alignment - if self.segdup_caller is not None: - out_segdup = pathlib.Path( - str(self.output_vcf).replace(".vcf.gz", "_segdups") - ) - genes = ",".join(self.segdup_caller) or None - segdup_job = self.build_segdup_job( - out_segdup, cnv_input_bams[0], genes - ) - dag.add_job(segdup_job, cnvscope_deps) - # Estimate ploidy when needed for sex-aware downstream tools - if self.expansion_catalog: + if self.expansion_catalog or self.segdup_caller is not None: ploidy_job = self.build_ploidy_job( self.ploidy_json, [cnv_input_bams[0]] ) @@ -1189,6 +1186,7 @@ def build_segdup_job( self, out_segdup: pathlib.Path, sr_alignment: pathlib.Path, + input_vcf: pathlib.Path, genes: Optional[str], ) -> Job: """Call variants in difficult SegDups""" @@ -1199,12 +1197,15 @@ def build_segdup_job( self.logger.error("model_bundle is required") sys.exit(2) + sex = "male" if self.sample_sex == SampleSex.MALE else "female" return Job( cmds.cmd_segdup_caller( out_segdup, sr_alignment, reference=self.reference, sr_bundle=self.model_bundle, + input_vcf=input_vcf, + sex=sex, genes=genes, ), "segdup-caller", @@ -1225,6 +1226,24 @@ def build_second_dag(self) -> DAG: ) dag.add_job(expansion_job) + # SegDup calling consumes the small-variant VCF and the inferred sex + if ( + self.segdup_caller is not None + and self.sr_alignment + and self.output_vcf + ): + out_segdup = pathlib.Path( + str(self.output_vcf).replace(".vcf.gz", "_segdups") + ) + genes = ",".join(self.segdup_caller) or None + segdup_job = self.build_segdup_job( + out_segdup, + self.sr_alignment, + self.output_vcf, + genes, + ) + dag.add_job(segdup_job) + return dag def build_t1k_jobs( From 93cdf1b95a5060fcb2a15493a4caeed393682fd0 Mon Sep 17 00:00:00 2001 From: Don Freed Date: Fri, 1 May 2026 11:58:35 -0700 Subject: [PATCH 19/22] Update the Dockerfile to add an ExpansionHunter wrapper Use sentieon `ReadWriter` to merge input BAM/CRAM files in the `dnascope-pangenome` pipeline instead of `samtools merge`. --- Dockerfile | 10 +++++-- sentieon_cli/command_strings.py | 52 ++++++++++++++++++--------------- sentieon_cli/driver.py | 6 ++-- 3 files changed, 40 insertions(+), 28 deletions(-) diff --git a/Dockerfile b/Dockerfile index 71a77f3..4e21342 100644 --- a/Dockerfile +++ b/Dockerfile @@ -229,6 +229,10 @@ COPY --from=downloader /opt/sentieon-cli/dist /opt/sentieon-cli/dist # /usr/lib) and for the unversioned names of the third-party tools. # `run-t1k` resolves its sibling binaries via abs_path($0), so symlinking # it from /usr/local/bin keeps the binaries discoverable in /opt/t1k-*/. +# `ExpansionHunter` goes through a wrapper that strips LD_PRELOAD: the +# pre-built EH binary crashes with `std::bad_cast` while parsing its JSON +# variant catalog when the global jemalloc preload (set further down) is +# active. RUN cd /usr/lib && \ ln -s libisal.so.2.0.30 libisal.so.2 && \ ln -s libisal.so.2 libisal.so && \ @@ -239,8 +243,10 @@ RUN cd /usr/lib && \ ln -s kmc-${KMC_VERSION} kmc && \ ln -s kmc_tools-${KMC_VERSION} kmc_tools && \ ln -s kmc_dump-${KMC_VERSION} kmc_dump && \ - ln -s ExpansionHunter-${EXPANSIONHUNTER_VERSION} ExpansionHunter && \ - ln -s /opt/t1k-${T1K_VERSION}/run-t1k run-t1k + ln -s /opt/t1k-${T1K_VERSION}/run-t1k run-t1k && \ + printf '#!/bin/sh\nexec env -u LD_PRELOAD /usr/local/bin/ExpansionHunter-%s "$@"\n' \ + "${EXPANSIONHUNTER_VERSION}" > ExpansionHunter && \ + chmod +x ExpansionHunter # Install runtime dependencies. RUN apt-get update && \ diff --git a/sentieon_cli/command_strings.py b/sentieon_cli/command_strings.py index b48936c..55a4d1b 100644 --- a/sentieon_cli/command_strings.py +++ b/sentieon_cli/command_strings.py @@ -12,7 +12,7 @@ import typing from typing import Any, Dict, List, Optional, Union -from .driver import BaseDriver +from .driver import BaseDriver, Driver, ReadWriter from .logging import get_logger from .shell_pipeline import Command, InputProcSub, Pipeline @@ -895,30 +895,36 @@ def cmd_extract_kmc( reference: pathlib.Path, extract_model: pathlib.Path, tmp_dir: pathlib.Path, - threads=1, + threads: int = 1, ) -> Pipeline: - merge_cmd = Command( - "samtools", - "merge", - "--reference", - str(reference), - "-@", - str(threads), - "-u", - "-o", - "/dev/stdout", - *[str(x) for x in input_aln], + rw_bam = tmp_dir.joinpath("extract-kmc-rw.bam") + + driver = Driver( + reference=reference, + thread_count=threads, + input=input_aln, ) - view_cmd = Command( - "samtools", - "view", - "-@", - str(threads), - "-ub", - "-F", - "0xf00", - "-", + driver.add_algo( + ReadWriter( + rw_bam, + bam_compression=1, + output_flag_filter="0xf00:0", + ) ) + driver_cmd_str = shlex.join(driver.build_cmd()) + + # ReadWriter cannot write to /dev/stdout; symlink rw_bam -> /dev/stdout + # so the driver's writes hit the pipe to pgutil. `exec` so signals/exit + # codes propagate from the driver instead of the wrapping shell. + readwriter_cmd = Command( + "sh", + "-c", + ( + f"ln -sf /dev/stdout {shlex.quote(str(rw_bam))} && " + f"exec {driver_cmd_str}" + ), + ) + extract_cmd = Command( "sentieon", "pgutil", @@ -949,7 +955,7 @@ def cmd_extract_kmc( str(output_prefix), str(tmp_dir), ) - return Pipeline(merge_cmd, view_cmd, extract_cmd, kmc_cmd) + return Pipeline(readwriter_cmd, extract_cmd, kmc_cmd) def cmd_vg_haplotypes( diff --git a/sentieon_cli/driver.py b/sentieon_cli/driver.py index 09d545f..c2bbb7f 100644 --- a/sentieon_cli/driver.py +++ b/sentieon_cli/driver.py @@ -271,9 +271,9 @@ def __init__( output_flag_filter: Optional[str] = None, ): self.output = output - bam_compression = bam_compression - cram_write_options = cram_write_options - output_flag_filter = output_flag_filter + self.bam_compression = bam_compression + self.cram_write_options = cram_write_options + self.output_flag_filter = output_flag_filter class InsertSizeMetricAlgo(BaseAlgo): From fa7cc0ced1b3db6bf9a6272455660e0a410220bc Mon Sep 17 00:00:00 2001 From: Don Freed Date: Fri, 1 May 2026 12:16:24 -0700 Subject: [PATCH 20/22] Update segdup-caller version in Dockerfile --- Dockerfile | 10 +++++++--- 1 file changed, 7 insertions(+), 3 deletions(-) diff --git a/Dockerfile b/Dockerfile index 4e21342..b47db01 100644 --- a/Dockerfile +++ b/Dockerfile @@ -26,9 +26,9 @@ ARG EXPANSIONHUNTER_SHA256=ebf3ec0ace6e6e3bbce12c26463da5d9f8e16374eff1ad10f0f1a ARG T1K_VERSION=1.0.9 ARG T1K_GIT_TAG=v1.0.9 ARG T1K_COMMIT=9376b555c1d8d2f8ca357c2656f49f450462dbc3 -ARG SEGDUP_CALLER_VERSION=0.5.0 -ARG SEGDUP_CALLER_GIT_TAG=v0.5.0 -ARG SEGDUP_CALLER_COMMIT=cc8ddfb7d8a18ff254c5126ec879e8a18ee28389 +ARG SEGDUP_CALLER_VERSION=0.5.1 +ARG SEGDUP_CALLER_GIT_TAG=v0.5.1 +ARG SEGDUP_CALLER_COMMIT=82bb80f65f28436c4d28c5aa17f99b4e36bd6fe9 # Pinned Poetry toolchain; should match the version used to generate poetry.lock. ARG POETRY_VERSION=2.3.4 ARG POETRY_PLUGIN_EXPORT_VERSION=1.9.0 @@ -291,4 +291,8 @@ RUN sentieon driver --help && \ ExpansionHunter --help && \ perl -c "$(command -v run-t1k)" && \ segdup-caller --version && \ + python -c "import sys; from packaging.version import Version; \ +from sentieon_cli.sentieon_pangenome import SEGDUP_MIN_VERSION; \ +from sentieon_cli.util import check_version; \ +sys.exit(0 if all(check_version(c, v) for c, v in SEGDUP_MIN_VERSION.items()) else 1)" && \ sentieon-cli -h From b2e2544fa9ce8ac05e61ba4bedd2ca2e173660d1 Mon Sep 17 00:00:00 2001 From: Don Freed Date: Fri, 1 May 2026 12:26:44 -0700 Subject: [PATCH 21/22] Bump version --- pyproject.toml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/pyproject.toml b/pyproject.toml index 24c5074..331f196 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -1,7 +1,7 @@ [project] name = "sentieon_cli" -version = "1.6.1" +version = "1.6.2" description = "Pipeline implementations for the Sentieon software" authors = [ {name = "Don Freed", email = "don.freed@sentieon.com"}, From 92167f53310b0e66491b01a61cd475f0c6a20fa8 Mon Sep 17 00:00:00 2001 From: Don Freed Date: Sat, 2 May 2026 20:43:53 -0700 Subject: [PATCH 22/22] Restructure bam-input pipeline in `dnascope-pangenome` - Update the docker container for segdup-caller --- Dockerfile | 5 +++-- sentieon_cli/command_strings.py | 22 ++++++---------------- sentieon_cli/sentieon_pangenome.py | 14 +++++++++++++- 3 files changed, 22 insertions(+), 19 deletions(-) diff --git a/Dockerfile b/Dockerfile index b47db01..800b254 100644 --- a/Dockerfile +++ b/Dockerfile @@ -28,7 +28,7 @@ ARG T1K_GIT_TAG=v1.0.9 ARG T1K_COMMIT=9376b555c1d8d2f8ca357c2656f49f450462dbc3 ARG SEGDUP_CALLER_VERSION=0.5.1 ARG SEGDUP_CALLER_GIT_TAG=v0.5.1 -ARG SEGDUP_CALLER_COMMIT=82bb80f65f28436c4d28c5aa17f99b4e36bd6fe9 +ARG SEGDUP_CALLER_COMMIT=0406ea78b7ff53d7a169b9aa945d7fcc7257ff12 # Pinned Poetry toolchain; should match the version used to generate poetry.lock. ARG POETRY_VERSION=2.3.4 ARG POETRY_PLUGIN_EXPORT_VERSION=1.9.0 @@ -269,9 +269,10 @@ ENV MALLOC_CONF=metadata_thp:auto,background_thread:true,dirty_decay_ms:30000,mu # only its CLI entry point is exposed on PATH. COPY --from=python-builder /opt/sentieon-cli-venv /opt/sentieon-cli-venv COPY --from=python-builder /opt/segdup-caller-venv /opt/segdup-caller-venv -RUN ln -s /opt/segdup-caller-venv/bin/segdup-caller /usr/local/bin/segdup-caller ENV VIRTUAL_ENV=/opt/sentieon-cli-venv ENV PATH=/opt/sentieon-cli-venv/bin:$PATH +# Append (not prepend) the segdup-caller venv +ENV PATH=$PATH:/opt/segdup-caller-venv/bin # Create a non-root user for running the pipelines RUN useradd --create-home --uid 1001 --shell /bin/bash sentieon diff --git a/sentieon_cli/command_strings.py b/sentieon_cli/command_strings.py index 55a4d1b..86d00bf 100644 --- a/sentieon_cli/command_strings.py +++ b/sentieon_cli/command_strings.py @@ -895,10 +895,12 @@ def cmd_extract_kmc( reference: pathlib.Path, extract_model: pathlib.Path, tmp_dir: pathlib.Path, + rw_bam: pathlib.Path, threads: int = 1, ) -> Pipeline: - rw_bam = tmp_dir.joinpath("extract-kmc-rw.bam") - + # `rw_bam` must already be a symlink to /dev/stdout; + # ReadWriter writes to it, which resolves to the driver's stdout (= the + # pipe to pgutil extract). driver = Driver( reference=reference, thread_count=threads, @@ -911,19 +913,7 @@ def cmd_extract_kmc( output_flag_filter="0xf00:0", ) ) - driver_cmd_str = shlex.join(driver.build_cmd()) - - # ReadWriter cannot write to /dev/stdout; symlink rw_bam -> /dev/stdout - # so the driver's writes hit the pipe to pgutil. `exec` so signals/exit - # codes propagate from the driver instead of the wrapping shell. - readwriter_cmd = Command( - "sh", - "-c", - ( - f"ln -sf /dev/stdout {shlex.quote(str(rw_bam))} && " - f"exec {driver_cmd_str}" - ), - ) + driver_cmd = Command(*driver.build_cmd()) extract_cmd = Command( "sentieon", @@ -955,7 +945,7 @@ def cmd_extract_kmc( str(output_prefix), str(tmp_dir), ) - return Pipeline(readwriter_cmd, extract_cmd, kmc_cmd) + return Pipeline(driver_cmd, extract_cmd, kmc_cmd) def cmd_vg_haplotypes( diff --git a/sentieon_cli/sentieon_pangenome.py b/sentieon_cli/sentieon_pangenome.py index 53d0bb5..b17d3da 100644 --- a/sentieon_cli/sentieon_pangenome.py +++ b/sentieon_cli/sentieon_pangenome.py @@ -658,6 +658,17 @@ def build_first_dag(self) -> DAG: haplotype_dependencies.add(bwa_job) else: dnascope_bams = copy.deepcopy(self.sample_input) + # ReadWriter cannot write to /dev/stdout directly; pre-create a + # symlink so the driver writes to a real path that resolves to + # its stdout (the pipe to pgutil extract). + rw_bam = self.tmp_dir.joinpath("extract-kmc-rw.bam") + ln_job = Job( + Pipeline(Command("ln", "-sf", "/dev/stdout", str(rw_bam))), + "extract-kmc-symlink", + 1, + ) + dag.add_job(ln_job) + extract_kmc_job = Job( cmds.cmd_extract_kmc( kmer_prefix, @@ -666,12 +677,13 @@ def build_first_dag(self) -> DAG: self.reference, self.model_bundle.joinpath("extract.model"), self.tmp_dir, + rw_bam, threads=self.cores, ), "extract-kmc", self.cores, ) - dag.add_job(extract_kmc_job) + dag.add_job(extract_kmc_job, {ln_job}) haplotype_dependencies.add(extract_kmc_job) # vg haplotypes - create a sample-specific pangenome