diff --git a/.changeset/inject-source-order-pre.md b/.changeset/inject-source-order-pre.md
new file mode 100644
index 000000000..372a1f6fd
--- /dev/null
+++ b/.changeset/inject-source-order-pre.md
@@ -0,0 +1,5 @@
+---
+'@tanstack/devtools-vite': patch
+---
+
+Run source injection before the transforms of other plugins (unless they also use `order: 'pre'`), also when `devtools()` is not first in the plugins array. Before this fix, a framework plugin that changed the code of only one environment shifted the line numbers, so `data-tsd-source` differed between SSR and client and caused hydration warnings.
diff --git a/packages/devtools-vite/src/plugin.ts b/packages/devtools-vite/src/plugin.ts
index 8c7acdd65..fd7260187 100644
--- a/packages/devtools-vite/src/plugin.ts
+++ b/packages/devtools-vite/src/plugin.ts
@@ -60,6 +60,12 @@ export const devtools = (args?: TanStackDevtoolsViteConfig): Array<Plugin> => {
         return config.mode === 'development' && injectSourceConfig.enabled
       },
       transform: {
+        // Run before the transforms of plugins without `order: 'pre'`, also when
+        // devtools() is not first in the plugins array. A plugin that changes
+        // the code of one environment only would shift the lines and make the
+        // SSR and client locations differ, which breaks hydration. An earlier
+        // plugin whose transform also has `order: 'pre'` still runs first.
+        order: 'pre',
         filter: {
           id: {
             exclude: [/node_modules/, /\?raw/, /\/dist\//, /\/build\//],
diff --git a/packages/devtools-vite/tests/inject-source-order.test.ts b/packages/devtools-vite/tests/inject-source-order.test.ts
new file mode 100644
index 000000000..67dce0913
--- /dev/null
+++ b/packages/devtools-vite/tests/inject-source-order.test.ts
@@ -0,0 +1,62 @@
+// @vitest-environment node
+import { mkdtempSync, rmSync, writeFileSync } from 'node:fs'
+import { tmpdir } from 'node:os'
+import { join } from 'node:path'
+import { createServer } from 'vite'
+import { afterAll, beforeAll, describe, expect, it } from 'vitest'
+import { devtools } from '../src/plugin'
+import type { ViteDevServer } from 'vite'
+
+describe('inject-source order', () => {
+  let root: string
+  let server: ViteDevServer
+
+  beforeAll(async () => {
+    root = mkdtempSync(join(tmpdir(), 'tsd-inject-order-'))
+    writeFileSync(
+      join(root, 'App.tsx'),
+      'export function App() {\n  return <div>hi</div>\n}\n',
+    )
+    server = await createServer({
+      root,
+      mode: 'development',
+      configFile: false,
+      logLevel: 'silent',
+      server: { middlewareMode: true, ws: false },
+      // The classic runtime needs no react import, so the fixture needs no
+      // node_modules.
+      oxc: { jsx: { runtime: 'classic' } },
+      plugins: [
+        // A framework plugin listed before devtools() that changes the code of
+        // one environment only, like a server-function compiler.
+        {
+          name: 'shift-ssr-lines',
+          enforce: 'pre',
+          transform(code, id, options) {
+            return options?.ssr && id.endsWith('.tsx')
+              ? `\n\n\n${code}`
+              : undefined
+          },
+        },
+        devtools({
+          eventBusConfig: { enabled: false },
+          consolePiping: { enabled: false },
+        }),
+      ],
+    })
+  })
+
+  afterAll(async () => {
+    await server?.close()
+    rmSync(root, { recursive: true, force: true })
+  })
+
+  it('injects the same source location on the client and the server', async () => {
+    const client = await server.transformRequest('/App.tsx')
+    const ssr = await server.transformRequest('/App.tsx', { ssr: true })
+    const sourceOf = (code = '') => code.match(/App\.tsx:\d+:\d+/)?.[0]
+
+    expect(sourceOf(client?.code)).toBe('App.tsx:2:10')
+    expect(sourceOf(ssr?.code)).toBe('App.tsx:2:10')
+  })
+})