diff --git a/datafusion/optimizer/src/push_down_filter.rs b/datafusion/optimizer/src/push_down_filter.rs
index f30b1187b7bca..619d53fb045fe 100644
--- a/datafusion/optimizer/src/push_down_filter.rs
+++ b/datafusion/optimizer/src/push_down_filter.rs
@@ -576,6 +576,15 @@ fn infer_join_predicates(
     predicates: &[Expr],
     on_filters: &[Expr],
 ) -> Result<Vec<Expr>> {
+    let join_type = join.join_type;
+
+    // Null-aware anti joins implement NOT IN semantics, where NULLs on the
+    // right side can affect the result. Inferring predicates across the join can
+    // incorrectly filter those NULLs.
+    if join_type == JoinType::LeftAnti && join.null_aware {
+        return Ok(vec![]);
+    }
+
     // Only allow both side key is column.
     let join_col_keys = join
         .on
@@ -587,8 +596,6 @@ fn infer_join_predicates(
         })
         .collect::<Vec<_>>();
 
-    let join_type = join.join_type;
-
     let mut inferred_predicates = InferredPredicates::new(join_type);
 
     infer_join_predicates_from_predicates(
diff --git a/datafusion/sqllogictest/test_files/not_in_null_pushdown.slt b/datafusion/sqllogictest/test_files/not_in_null_pushdown.slt
new file mode 100644
index 0000000000000..d2ef490d6813f
--- /dev/null
+++ b/datafusion/sqllogictest/test_files/not_in_null_pushdown.slt
@@ -0,0 +1,48 @@
+# Licensed to the Apache Software Foundation (ASF) under one
+# or more contributor license agreements.  See the NOTICE file
+# distributed with this work for additional information
+# regarding copyright ownership.  The ASF licenses this file
+# to you under the Apache License, Version 2.0 (the
+# "License"); you may not use this file except in compliance
+# with the License.  You may obtain a copy of the License at
+#
+#   http://www.apache.org/licenses/LICENSE-2.0
+#
+# Unless required by applicable law or agreed to in writing,
+# software distributed under the License is distributed on an
+# "AS IS" BASIS, WITHOUT WARRANTIES OR CONDITIONS OF ANY
+# KIND, either express or implied.  See the License for the
+# specific language governing permissions and limitations
+# under the License.
+
+# Regression test for NOT IN null-aware semantics. The right-side NULL must not
+# be removed by inferred predicate pushdown through the null-aware anti join.
+
+statement ok
+CREATE TABLE pushdown_t(id INT, a INT)
+
+statement ok
+INSERT INTO pushdown_t VALUES (1, 1), (2, 2)
+
+statement ok
+CREATE TABLE pushdown_u(k INT)
+
+statement ok
+INSERT INTO pushdown_u VALUES (NULL)
+
+# Expected result is empty:
+# - id=1: a <> 1 is false
+# - id=2: a <> 1 is true, but 2 NOT IN (NULL) is UNKNOWN
+# WHERE only keeps TRUE, so neither row should be returned.
+query I
+SELECT id
+FROM pushdown_t
+WHERE a != 1
+  AND a NOT IN (SELECT k FROM pushdown_u);
+----
+
+statement ok
+DROP TABLE pushdown_t
+
+statement ok
+DROP TABLE pushdown_u