Light and dark are the cycle, not a setting (#183) - #538
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Synechococcus_Shewanella_Dlactate_Biophotovoltaic_Consortium: dual-chamber electrochemical devices on M9, run for over 40 days. The cyanobacterium charges the system in the light by making d-lactate; the engineered Shewanella discharges it in the dark by oxidising that d-lactate to the anode. Illumination is deliberately not a field. Charging and discharging run sequentially under light and dark, and the paper's own attempt at continuous light produced no current at all — photosynthetic oxygen raised dissolved oxygen, and Shewanella is itself light-sensitive. A single illumination value would turn that finding into a parameter. No applied_potential either: the paper reports power output, not poised- potential operation, so the electrode is described rather than given a voltage it never states. The LB at 30 °C in the same paper is the S. oneidensis preculture and is not here — the third record in this batch where the community's conditions and a member's sit side by side (#529). Co-Authored-By: Claude Opus 5 <noreply@anthropic.com>
Network integrity findingsWarnings only — a member with no interaction yet, or a participant matched by ontology id rather than by name, or one on a community-level interaction that resolves to no member. Reported, but does not fail the build. The full report is attached to the workflow run as an artifact. |
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What
Synechococcus_Shewanella_Dlactate_Biophotovoltaic_Consortium— dual-chamber electrochemical devices on M9 medium, operated for over 40 days.The cyanobacterium charges the system in the light by making d-lactate; the engineered S. oneidensis discharges it in the dark by oxidising that d-lactate to the anode.
Illumination is deliberately not a field
Charging and discharging run sequentially under light and dark. And the paper's own attempt to run the consortium under continuous light produced no current at all — photosynthetic oxygen raised dissolved oxygen, and S. oneidensis is itself light-sensitive, with cell death after 24 h of illumination.
Recording a single illumination value would turn that result into a parameter. The light/dark alternation is the operating cycle, so it lives in
instrument_detailandnoteswith the snippet that states it.Two other fields left empty
applied_potential— the paper reports power output (>150 mW·m⁻²), not poised-potential operation. The electrode is described rather than given a voltage the source never states.cultivation_modeisBATCHbecause the devices are charged and discharged rather than fed and drained; no dilution or feed rate is reported.Preculture excluded again
The LB at 30 °C elsewhere in the paper is the S. oneidensis preculture, not the consortium. That is the third record in this batch where a member's conditions sit beside the community's in the same source (#529) — and, as before, the member-coverage guard was green and the discrimination came from reading which sentence the number attaches to.
Here the supporting snippet resolves it directly: the dual-chamber sentence says the devices were used for "mono-cultures and microbial consortia in this study."
Checks
just validate— no issuesjust validate-references— 0 issues; all three snippets verbatimjust validate-strict,just lint,just check-docs-current— exit 0uv run pytest tests/— 2375 passed, 16 skippedPart of #183.
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