The amendments are the coculture, not a treatment on it (#183)

kb/communities/Dehalococcoides_Pelobacter_Acetylene_TCE_Coculture.yaml

@@ -227,6 +227,42 @@ ecological_interactions:
     evidence_source: IN_VITRO
     snippet: inhibition was removed by adding Pelobacter SFB93
     explanation: Supports SFB93 as a functional partner that can relieve acetylene inhibition.
+cultivation_setup:
+- cultivation_mode: BATCH
+  system_type: SERUM_BOTTLE
+  working_volume: 25.0
+  working_volume_unit: mL
+  operating_temperature: 34.0
+  operating_temperature_unit: °C
+  instrument_detail: >-
+    60 mL serum bottles holding 25 mL of artificial bay water medium under an N2 headspace,
+    static, each strain inoculated at 10% v/v. TCE and acetylene were both supplied at
+    0.1 mM - the acetylene is the electron donor and carbon source for Pelobacter, which is
+    what feeds the dechlorinating partner, so the two amendments together are the coculture
+    rather than a treatment applied to it.
+  notes: >-
+    Static on purpose, as with the thermophilic cellulose coculture: these are strict
+    anaerobes on volatile substrates in a sealed bottle, and agitation would work against
+    both the gas balance and the syntrophic exchange.
+
+    Cocultures were carried forward by 5% v/v transfer once 20 μmol TCE had gone to vinyl
+    chloride and 36 μmol acetylene was depleted, so the batch is serial rather than
+    single-shot; that is in the description rather than compressed into cultivation_mode,
+    which has no value for it.
+
+    The monoculture conditions in the same paper - strain 195 and BAV1 on defined medium
+    under H2/CO2 (80:20), SFB93 alone on ABW - are not recorded here (#529).
+  evidence:
+  - reference: PMID:28075122
+    supports: SUPPORT
+    evidence_source: IN_VITRO
+    snippet: Bacterial cocultures of strains 195 and SFB93 (10% vol/vol inoculation of each bacterium)
+      were initially established in 60 mL serum bottles containing 25 mL ABW medium with TCE supplied
+      at a liquid concentration of 0.1 mM (corresponding to 3 μmol TCE per bottle), 0.1 mM acetylene
+      (corresponding to 0.15 mL gas), and N2 headspace incubated at 34 °C without agitation
+    explanation: Vessel, working volume, medium, amendments, headspace, temperature and static
+      operation - stated for the coculture itself.
+
 environmental_factors:
 - name: Acetylene co-contaminant
   value: acetylene generated during TCE degradation