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35 changes: 35 additions & 0 deletions kb/communities/Rifle_Aquifer_Bioanode_EET_Community.yaml
Original file line number Diff line number Diff line change
Expand Up @@ -280,6 +280,41 @@ ecological_interactions:
in the Rifle aquifer that may have the potential to mediate mineral redox
transformations
explanation: Supports the selective enrichment of EET-capable organisms.
cultivation_setup:
- system_type: BIOELECTROCHEMICAL_SYSTEM
instrument_detail: >-
Electroactive biofilms grown on poised anodes and maintained for almost four years, with
acetate as the sole carbon source. Both the anode biofilm and the planktonic fraction
were sampled, so the electrode is not merely a probe - it is the growth surface that
selects the community this record describes.
electrode_detail: >-
Anodes poised mostly at -0.2 to -0.25 V versus the standard hydrogen electrode, a range
chosen to mimic the redox potential of iron-oxide minerals.
notes: >-
`applied_potential` is left unset and the value kept in `electrode_detail`. The source
gives a range rather than a setpoint, and qualifies it with "mostly", so a single number
would assert a poise the four-year run did not hold.

This is also the case #517 anticipated: the potential is only meaningful against its
reference electrode, and "vs SHE" is not a unit. Keeping the reference in
`electrode_detail` is what lets the range be stated in full rather than compressed into
a value-plus-suffix.

No temperature or working volume - the source characterises the communities and does not
restate the reactor's dimensions.

Predicted curatable in #543 on the grounds that a bioanode implies an electrochemical
cell, and it is: the biofilms were established and maintained rather than sampled from
the aquifer directly.
evidence:
- reference: PMID:32849356
supports: SUPPORT
evidence_source: IN_VITRO
snippet: Electroactive biofilms were established and maintained for almost 4 years on anodes
poised mostly at -0.2 to -0.25 V vs. SHE, a range that mimics the redox potential of
iron-oxide minerals, using acetate as the sole carbon source
explanation: The electrode poise and its reference, the duration, and the sole carbon source.

environmental_factors:
- name: Anode redox potential
value: -0.2 to -0.25 V vs SHE
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