Info

Collection of sequencing adapter/primer sequences (mainly Illumina) from all around the internet. I appologize but this list is so old I no longer remember most of the sources where I got it from.

Each adapter has it's reverse-complement "version" at the end since I've observed this "version" in the past at some experiments.

The code to merge & deduplicate sequences is from Stephen Turner.

Notes

Make sure your adapter fasta is one-line fasta:

cat adapters.fa | awk '/^>/ { if(NR>1) print "";  printf("%s\n",$0); next; } { printf("%s",$0);}  END {printf("\n");}' > adapters-oneline.fa

Merge and deduplicate with combine_adapters.R:

R --no-save < combine_adapters.R

The adapter file/files have to be in this directory (doesn't go recursive) and must have .fa (not .fasta) suffix.

Sources

• https://github.com/OpenGene/fastp/blob/master/src/knownadapters.h
• https://github.com/stephenturner/adapters/blob/master/adapters_combined_256_unique.fasta
• https://github.com/BioInfoTools/BBMap/blob/master/resources/adapters.fa
• Other sources I have forgotten about (I apologize).